Bone induction through controlled release of novel BMP-2-related peptide from PTMC11-F127-PTMC11 hydrogels
Bone induction through controlled release of novel BMP-2-related peptide from PTMC11-F127-PTMC11 hydrogels
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DOI：
10.1088/1748-6041/7/1/015008
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Guo, Xiaodong
中科院分区：
文献类型：
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作者：
Tang, Shuo;Zhao, Jingjing;Guo, Xiaodong
骨形态发生蛋白2（BMP-2）是最强大的成骨因子；其增强成骨细胞活化的功效已在体外和体内得到证实。我们开发了一种源自 BMP-2 的“指关节”表位的新型肽（命名为 P24），并发现它在一定程度上也具有成骨生物活性。本研究的主要目的是开发一种基于聚（三亚甲基碳酸酯）-F127-聚（三亚甲基碳酸酯）（PTMC11-F127-PTMC11）水凝胶的P24肽控释系统，以促进骨形成。通过改变共聚物浓度，我们证明 P24/PTMC11-F127-PTMC11 水凝胶是 P24 在 21-35 天内持续释放的有效系统。负载 P24 的水凝胶可提高体外骨髓基质细胞 (BMSC) 中的碱性磷酸酶活性并促进骨钙素 mRNA 的表达。放射照相和组织学检查表明，负载 P24 的水凝胶比不含 P24 的水凝胶可以更有效地诱导体内异位骨形成。这些结果表明PTMC11-F127-PTMC11水凝胶是P24控释的合适载体，并且是一种有前途的用于诱导骨再生的可注射生物材料。
Bone morphogenetic protein 2 (BMP-2) is the most powerful osteogenic factor; its effectiveness in enhancing osteoblastic activation has been confirmed both in vitro and in vivo. We developed a novel peptide (designated P24) derived from the 'knuckle' epitope of BMP-2 and found it also had osteogenic bioactivity to some extent. The main objective of this study was to develop a controlled release system based on poly(trimethylene carbonate)-F127-poly(trimethylene carbonate) (PTMC11-F127-PTMC11) hydrogels for the P24 peptide, to promote bone formation. By varying the copolymer concentrations, we demonstrated that P24/PTMC11-F127-PTMC11 hydrogels were an efficient system for the sustained release of P24 over 21-35 days. The P24-loaded hydrogels elevated alkaline phosphatase activity and promoted the expression of osteocalcin mRNA in bone marrow stromal cells (BMSCs) in vitro. Radiographic and histological examination showed that P24-loaded hydrogels could induce more effective ectopic bone formation in vivo than P24-free hydrogels. These results indicate that the PTMC11-F127-PTMC11 hydrogel is a suitable carrier for the controlled release of P24, and is a promising injectable biomaterial for the induction of bone regeneration.