Micropropagation of switchgrass by node culture
Micropropagation of switchgrass by node culture
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DOI：
10.2135/cropsci1996.0011183x003600060049x
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发表时间：
1996-11-01
期刊：
影响因子：
2.3
通讯作者：
Conger, BV
中科院分区：
文献类型：
--
作者：
Alexandrova, KS;Denchev, PD;Conger, BV
柳枝草(Panicum virgatum L.)是天然的异交；因此，很难通过有性繁殖来维持基因。这项研究的目的是开发一种微繁殖程序，用于所需或选定的基因类型的增殖。节段表面灭菌，纵裂，切面放置在含有30g L麦芽糖和不同浓度的6-苄氨基嘌呤的固体MS培养基上。不定芽增殖以12.5亩M-BAP+29℃培养效果最好，不定芽在不含BAP的MS培养基上容易生根。在最佳条件下，一株亲本在12wk内可生产出约500株植株。
Switchgrass (Panicum virgatum L.) is naturally outcrossing; therefore, maintenance of genotype is difficult through sexual propagation. The objective of this study was to develop a micropropagation procedure for the multiplication of desired or selected genotypes. Nodal segments were surface sterilized, split Longitudinally, and placed with the cut surface on solid MS medium containing 30 g L(-1) maltose and different concentrations of 6-benzylaminopurine (BAP) as the only growth regulator. Best shoot proliferation was obtained with 12.5 mu M BAP and culture at 29 degrees C. Shoots were easily rooted on MS medium without BAP. Under optimum conditions, it is possible to produce approximately 500 plantlets from one parent plant in 12 wk.