An "on-off" electrochemiluminescence immunosensor for PIVKA-II detection based on the dual quenching of CeO2-Au-g-C3N4 hybrids by Ag nanocubes-VB2
An "on-off" electrochemiluminescence immunosensor for PIVKA-II detection based on the dual quenching of CeO2-Au-g-C3N4 hybrids by Ag nanocubes-VB2
复制标题
基于Ag纳米立方体-VB2对CeO2-Au-g-C3N4杂化物的双重猝灭的用于PIVKA-II检测的“开关”电化学发光免疫传感器
DOI：
10.1016/j.bios.2021.113059
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发表时间：
2021-02-06
影响因子：
12.6
通讯作者：
Tang, Hua
中科院分区：
文献类型：
--
作者：
Ai, Zhujun;Zhao, Min;Tang, Hua
本文报道了一种基于ECL共振能量转移（ECL-RET）的新型双猝灭电化学发光（ECL）免疫传感器，用于检测维生素K缺乏或拮抗剂II（PIVKA-II）诱导的蛋白质。本实验制备了CeO 2和Au纳米粒子功能化的g-C3 N4纳米片（CeO 2-Au-g-C3 N4）的自加速电化学发光杂化材料，该材料在S2 O 82-作为共反应物时表现出高的电化学发光发射。CeO 2与Ce 3+和Ce 4+之间的氧化还原电对可以作为一种有效的共反应促进剂，产生更多的氧化中间体（SO 4中心点-），从而显著地自促进g-C3 N4 NS/S2 O 82- ECL体系的ECL发射。此外，Au纳米粒子不仅加速了电化学发光过程中的电子传递，而且为生物分子的固定提供了大量的活性位点。首次提出了维生素B-2修饰的银纳米立方体（AgNCs-VB 2）对g-C3 N4 NS/S2 O 82- ECL体系的双猝灭标记，使“信号关”态的ECL显著降低。基于夹心免疫反应原理，“on-off”型PIVKA-II ECL免疫传感器具有良好的检测灵敏度，线性范围为0.4 pg mL（-1）~ 10 ng mL（-1），检测下限为28.46 fg mL（-1）（S/N = 3）。该策略可能为医学研究和肝细胞癌早期临床诊断中的PIVKA-II检测提供潜在的替代工具。
Herein, we report a novel dual-quenching electrochemiluminescence (ECL) immunosensor for detecting protein induced by vitamin K absence or antagonist-II (PIVKA-II) based on ECL resonance energy transfer (ECL-RET). In this protocol, self-accelerated ECL hybrids of CeO2 and Au nanoparticles functionalized g-C3N4 nanosheets (CeO2-Au-g-C3N4) were prepared, which exhibited high ECL emission in the presence of S2O82- as a coreactant for "signal on" state. Concretely, CeO2 with a reproducible redox couple of Ce3+ and Ce4+ could act as an efficient co-reaction accelerator to generate more oxidizing intermediate (SO4 center dot-) to significantly self-promote the ECL emission of g-C3N4 NSs/S2O82- ECL system. Besides, Au nanoparticles not only accelerated electron transfer in the ECL process, but also provided massive active sites for biomolecules immobilization. The dual quenching labels of Ag nanocubes modified with vitamin B-2 (AgNCs-VB2) were firstly proposed towards g-C3N4 NSs/S2O82- ECL system by ECL-RET, resulting in the remarkable ECL decrease for "signal off" state. Based on the sandwich immunoreaction, the "on-off' PIVKA-II ECL immunosensor gratifyingly possessed excellent detection sensitivity with the linear range of 0.4 pg mL(-1) -10 ng mL(-1) and the low detection limit of 28.46 fg mL(-1) (S/N = 3). This presented strategy might provide a potential alternative tool for PIVKA-II detection in medical research and early clinical diagnostics of hepatocellular carcinoma.