Polymerase chain reaction protocols for rabies virus discrimination
Polymerase chain reaction protocols for rabies virus discrimination
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DOI：
10.1016/s0166-0934(98)00106-2
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发表时间：
1998-11-01
影响因子：
3.1
通讯作者：
Nadin-Davis, SA
中科院分区：
文献类型：
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作者：
Nadin-Davis, SA
RT-PCR方法的发展极大地促进了许多狂犬病病毒(RV)的遗传特征的研究，这些狂犬病病毒的不同毒株存在于地理区域内的某些宿主物种储藏库中。轮状病毒基因组某些区域的相对时间保守性，特别是N基因，使快速分子方法的发展成为可能。病毒鉴别主要有两种方法：(1)聚合酶链式反应产物的限制性片段长度多态(RFLP)；(2)毒株特异性聚合酶链式反应(SS-PCR)，即利用毒株特异性引物对特定毒株的序列进行差异扩增。这两种方法都为安大略省的狂犬病流行病学研究和控制计划提供了有价值的方法。这些程序有助于鉴定北极狐的株内变异，这是该地区唯一存在的陆生RV株，它们使人们能够快速区分这种株与食虫蝙蝠水库中流行的株以及外来浣熊株，后者继续在美国东北部传播，并有可能进入安大略省。这种方法可以很容易地适用于拥有多个重叠的房车储集层的其他地区。(C)1998由爱思唯尔科学爱尔兰有限公司出版。保留所有权利。
The development of RT-PCR methodology has facilitated greatly the genetic characterisation of many rabies viruses (RVs), distinct strains of which persist in certain host species reservoirs within geographically defined regions. The relative temporally conserved nature of Certain regions of the RV genome, particularly the N gene, permits development of rapid molecular methods for RV typing. Two main strategies have been applied to viral discrimination: (I) restriction fragment length polymorphism (RFLP) of PCR products and (2) strain-specific PCR (SS-PCR), in which sequences of specific viral strains are amplified differentially using strain-specific primers. Both these approaches have yielded methods of value to rabies epidemiological studies and control programs in Ontario. These procedures have facilitated the identification of intra-strain variants of the arctic fox strain, the only terrestrial RV strain persisting in the area, and they allow rapid discrimination of this strain from those circulating in insectivorous bat reservoirs and from the foreign raccoon strain, which continues to spread throughout the northeastern US and threatens to enter Ontario. Such methods can be adapted readily for use in other regions harbouring multiple overlapping RV reservoirs. (C) 1998 Published by Elsevier Science Ireland Ltd. All rights reserved.