Polydiacetylene Nanofiber Composites as a Colorimetric Sensor Responding To Escherichia coli and pH.
Polydiacetylene Nanofiber Composites as a Colorimetric Sensor Responding To Escherichia coli and pH.
复制标题
DOI：
10.1021/acsomega.7b01136
复制
发表时间：
2017-10-31
期刊：
影响因子：
4.1
通讯作者：
Li YV
中科院分区：
文献类型：
--
作者：
Yapor JP;Alharby A;Gentry-Weeks C;Reynolds MM;Alam AKMM;Li YV
聚二乙炔（pda）是一种共轭聚合物，其颜色变化是对外界刺激的反应。本研究将10,12-五甲酸（PCDA）与聚环氧乙烷（PEO）和聚氨酯（PU）等载体聚合物混合，采用静电纺丝法制备纤维复合材料。采用紫外光聚合法制备PEO-PDA和PU-PDA纳米纤维垫，纤维直径在130 nm ~ 2.5 μm之间。PEO - pda和PU - pda纳米纤维的形貌均与静电纺丝参数（PCDA / PEO或PCDA / PU）和总聚合物浓度有关。扫描电镜图像显示PEO-PDA和PU-PDA分别在2和18 w/v %浓度下呈串珠状纤维。当PEO-PDA的溶剂浓度增加到3.75 w/v %， PU-PDA的溶剂浓度增加到25 w/v %时，纤维变得光滑。PEO-PDA和PU-PDA纳米纤维复合材料对大肠杆菌ATCC25922细菌细胞的存在和pH值的变化均表现出良好的比色响应。当将纳米纤维直接暴露于培养在Luria-Bertani琼脂上的大肠杆菌ATCC25922时，纳米纤维表现出快速的比色反应。PEO - PDA和PU-PDA的比较表明，PEO和PDA的组合是有利的，因为它对大肠杆菌的存在提供了敏感的反应。结果与没有基质聚合物的PDA聚合物样品进行了比较。当pH值发生变化时，PDA聚合物和PDA纳米纤维复合材料的比色响应相似，pH值在10时发生颜色变化，pH值在11-13时增强。含PDA的纳米纤维复合材料比仅含PDA聚合物的纳米纤维具有更强的比色响应，表明其在生物传感器和化学传感器方面的潜力。
Polydiacetylenes (PDAs) are conjugative polymers that demonstrate color changes as a response to an external stimulus. In this study, 10,12-pentacosadiynoic acid (PCDA) was mixed with a supporting polymer including poly(ethylene oxide) (PEO) and polyurethane (PU), and the mixture solution was electrospun to construct fiber composites. The electrospun fibers were then photopolymerized using UV irradiation to produce PEO–PDA and PU–PDA nanofiber mats with a fiber diameter ranging from 130 nm to 2.5 μm. The morphologies of both PEO–PDA and PU–PDA nanofibers were dependent on electrospinning parameters such as the ratio of PCDA to PEO or PCDA to PU and the total polymer concentrations. Scanning electron microscopy images showed beaded fibers of PEO–PDA and PU–PDA at 2 and 18 w/v % concentrations, respectively. Smooth fibers were found when the solvent concentration was increased to 3.75 w/v % in PEO–PDA and 25 w/v % in PU–PDA fibers. Both PEO–PDA and PU–PDA nanofiber composites demonstrated excellent colorimetric responses to the presence of Escherichia coli ATCC25922 bacterial cells and the changes in pH as external stimuli. The nanofibers underwent a rapid colorimetric response when exposed directly to E. coli ATCC25922 grown on Luria–Bertani agar. The comparison between the PEO–PDA and PU–PDA suggested that the combination of PEO and PDA is favorable because it provides a sensitive response to the presence of E. coli. The results were compared with samples of a PDA polymer in the absence of a matrix polymer. The colorimetric response was similar when the PDA polymer and the PDA nanofiber composites were exposed to pH changes, and the color change was found to occur at pH 10 and enhanced at pH 11–13. The PDA-containing nanofiber composites showed stronger colorimetric responses than those of the PDA polymer only, suggesting their potential as biosensors and chemosensors.
登录
查看更多内容
影响因子：
4.6
作者：
Nie, Huarong;He, Aihua;Han, Charles C.
通讯作者：
Han, Charles C.
影响因子：
15
作者：
Ahn, DJ;Chae, EH;Kim, JM
通讯作者：
Kim, JM
影响因子：
9.5
作者：
Park, In Sung;Park, Hye Jin;Kim, Jong-Man
通讯作者：
Kim, Jong-Man
掺入 Fe3O4 纳米颗粒的磁致变色聚二乙炔
影响因子：
16.6
作者：
Chen, Xuli;Li, Li;Peng, Huisheng
通讯作者：
Peng, Huisheng
影响因子：
--
作者：
Lee, KH;Kim, HY;Choi, SW
通讯作者：
Choi, SW