Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.
Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.
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DOI：
10.1186/s12944-018-0896-0
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发表时间：
2018-11-06
影响因子：
4.5
通讯作者：
Xue Y
中科院分区：
文献类型：
--
作者：
Zeng Y;Guan M;Li C;Xu L;Zheng Z;Li J;Xue Y
苦瓜（Momentariacharantia，BM）是治疗糖尿病、高血压、血脂异常等代谢性疾病的有效补充剂，但苦瓜是否能预防动脉粥样硬化的进展尚不清楚。为了证实BM在动脉粥样硬化中的作用并探讨其潜在机制，我们设计了这项研究。将24只8周龄雄性ApoE基因敲除（ApoE-/-）小鼠随机分为对照组和BM组，每组10只。16周后测定大鼠体重、摄食量、血糖、血脂，分析动脉粥样硬化斑块面积及其组织学成分。采用定量聚合酶链反应检测血管细胞粘附分子和炎性细胞因子的表达。BM组体重增长和血清甘油三酯（TG）显著降低。BM不仅能降低动脉粥样硬化斑块面积和斑块内胶原纤维含量，还能降低血清可溶性血管细胞粘附分子（VCAM）-1和P-选择素水平，以及单核细胞趋化蛋白（MCP）-1和IL-6的表达。我们的研究表明，膳食BM可以减轻ApoE-/-小鼠动脉粥样硬化的发展，可能通过降低甘油三酯和抗炎机制。
Bitter melon (BM, Momordica charantia) has been accepted as an effective complementary treatment of metabolic disorders such as diabetes, hypertension, dyslipidemia and etc. However it is unclear whether BM can prevent the progression of atherosclerosis. To confirm the effects of BM on atherosclerosis and explore its underlying mechanisms, we design this study. Twenty four male apolipoprotein E knock-out (ApoE-/-) mice aged 8 weeks were randomly divided into control group fed with high fat diet (HFD) only and BM group fed with HFD mixed with 1.2%w/w BM. After 16 weeks, body weight, food intake, blood glucose, serum lipids were measured and the atherosclerotic plaque area and its histological composition were analyzed. The expression of vascular cell adhesive molecules and inflammatory cytokines in the aortas were determined using quantitative polymerase chain reaction. Body weight gain and serum triglycerides (TG) significantly decreased in BM group. BM reduced not only the atherosclerotic plaque area and the contents of collagen fibers in atherosclerotic plaques but also the serum soluble vascular cell adhesion molecule (VCAM)-1 and P-selectin levels, as well as the expressions of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-6 in aortas. Our study indicates that dietary BM can attenuate the development of atherosclerosis in ApoeE-/- mice possibly through reducing triglyceride and anti-inflammation mechanism.