Substrate independent ATPase activity may complicate high throughput screening.
Substrate independent ATPase activity may complicate high throughput screening.
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DOI：
10.1016/j.ab.2016.07.016
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发表时间：
2016-10-01
影响因子：
2.9
通讯作者：
Fung LW
中科院分区：
文献类型：
--
作者：
Tuntland ML;Fung LW
Inorganic phosphate release, [Pi], is often measured in an enzymatic reaction in a high throughput setting. Based on the published mechanism, we designed a protocol for our screening for inhibitors of SAICAR synthetase (PurC), and we found a gradual increase in [Pi] in positive control samples over the course of the day. Further investigation indicated that hydrolysis of ATP catalyzed by PurC, rather than substrate-related phosphate release, was responsible for a partial contribution to the signals in the control samples. Thus substrate-independent ATPase activity may complicate high throughput screening.
