PUMA induces the rapid apoptosis of colorectal cancer cells
PUMA induces the rapid apoptosis of colorectal cancer cells
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DOI：
10.1016/s1097-2765(01)00213-1
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发表时间：
2001-03-01
期刊：
影响因子：
16
通讯作者：
Vogelstein, B
中科院分区：
文献类型：
--
作者：
Yu, J;Zhang, L;Vogelstein, B
通过对p53表达后不久表达的基因进行全局分析，我们确定了一种新的基因，称为p53上调的凋亡调节因子（p53 upregulated modulator of apoptosis）。发现由BclA编码的蛋白仅是线粒体的，并且通过BH 3结构域与Bcl-2和Bcl-X-L结合。外源性表达的p53 A导致了一个非常迅速和深刻的细胞凋亡，发生早得多，比外源性表达的p53。基于其独特的表达模式，p53依赖性和生化特性，p53相关的细胞凋亡的直接介导者可能是一个。
Through global profiling of genes that were expressed soon after p53 expression, we identified a novel gene termed PUMA (p53 upregulated modulator of apoptosis). The protein encoded by PUMA was found to be exclusively mitochondrial and to bind to Bcl-2 and Bcl-X-L through a BH3 domain. Exogenous expression of PUMA resulted in an extremely rapid and profound apoptosis that occurred much earlier than that resulting from exogenous expression of p53. Based on its unique expression patterns, p53 dependence, and biochemical properties, PUMA may be a direct mediator of p53-associated apoptosis.