Tumor-infiltrating lymphocytes predict survival outcomes in patients with cervical cancer treated with concurrent chemoradiotherapy
Tumor-infiltrating lymphocytes predict survival outcomes in patients with cervical cancer treated with concurrent chemoradiotherapy
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DOI：
10.1016/j.ygyno.2020.07.106
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发表时间：
2020-11-01
影响因子：
4.7
通讯作者：
Aoki, Daisuke
中科院分区：
文献类型：
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作者：
Ohno, Akiko;Iwata, Takashi;Aoki, Daisuke
目标.为了（i）确定选定的免疫原性因素与临床病理学特征之间的相关性，（ii）确定肿瘤内各种特异性肿瘤浸润淋巴细胞（TIL）的丰度是否是接受基于顺铂的同步放化疗（CCRT）治疗的II期和III期宫颈癌女性的预后指标，研究15例宫颈癌新鲜标本中FOXP 3 + T细胞的亚型。在这项回顾性研究中，对55例晚期宫颈癌妇女阴道镜活检的肿瘤内病变进行了自动免疫染色，使用以下6种小鼠单克隆抗体：抗CD 3、抗CD 4、抗CD 8、抗CD 20、抗CD 206和抗FOXP 3。分析了每个标本中每种类型的TIL数量的自动评分结果与各种临床病理学特征之间的关联，以及各种特定类型的TIL丰度与生存率之间的关联。另外15例新鲜肿瘤标本中FOXP 3（+）TIL的亚型也采用流式细胞术进行了研究。CD 8（+）TIL浸润与盆腔淋巴结转移有关。CD 3（+）、CD 4（+）、CD 8（+）、CD 206（+）和FOXP 3（+）TIL的大量浸润是无进展生存期和总生存期较好的统计学显著指标。关于FOXP 3（+）TILs的亚型，在所有检测的样本中均发现非Tcl 3（Fr-III）。多种特异性肿瘤内TIL的丰度可能是接受CCRT的晚期宫颈癌患者的预后指标。(C)2020爱思唯尔公司All rights reserved.
Objectives. To (i) identify correlations between selected immunogenic factors and clinicopathological characteristics, (ii) determine whether intratumoral abundance of various specific tumor-infiltrating lymphocytes (TILs) is a prognostic indicator in women with Stage II and III cervical cancer who undergo treatment with cisplatin-based concurrent chemoradiotherapy (CCRT), and (iii) investigate subtypes of FOXP3+ T cells in 15 fresh samples of cervical cancer.Methods. In this retrospective study, intratumoral lesions in colposcopic biopsies from 55 women with advanced cervical cancer who subsequently underwent CCRT at our institution were subjected to automatic immunological staining using the following six mouse monoclonal antibodies: anti-CD3, anti-CD4, anti-CD8, antiCD20, anti-CD206, and anti-FOXP3. Associations between the findings on automatic scoring of the number of each type of TIL in each specimen and various clinicopathological characteristics were analyzed, as were associations between the abundance of various specific types of TIL and survival. Subtypes of FOXP3(+) TILs in 15 additional fresh tumor samples were also investigated using flow cytometry.Results. Infiltration with CD8(+) TILs was associated with pelvic lymph node metastasis. Abundant infiltration by CD3(+), CD4(+), CD8(+), CD206(+), and FOXP3(+) TILs were statistically significant indicators of better progressionfree and overall survival. Regarding subtypes of FOXP3(+) TILs, non-Tregs (Fr-III) were found in all samples tested for this.Conclusions. The abundance of various specific intratumoral TILs may be prognostic indicators in patients with advanced cervical cancer undergoing CCRT. (C) 2020 Elsevier Inc. All rights reserved.