CHARACTERIZATION AND SEPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION PRESENT IN URINE
CHARACTERIZATION AND SEPARATION OF AN INHIBITOR OF VIRAL HEMAGGLUTINATION PRESENT IN URINE
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DOI：
10.3181/00379727-74-17825
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发表时间：
1950-01-01
影响因子：
--
通讯作者：
HORSFALL, FL
中科院分区：
文献类型：
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作者：
TAMM, I;HORSFALL, FL
正常人尿液中含有一种高效的病毒血凝抑制物，对PR8、Lee、FM1、猪、流行性腮腺炎、新城疫和GDVII病毒有效，但对PVM无效。猫、兔和豚鼠的尿液也显示出抑制活性。抑制物浓缩。约为2毫克。%。通过3种步骤从人尿中分离出该抑制剂：乙醇沉淀法、pH 3沉淀法和0.58NaC l沉淀法。进一步纯化后，该物质的活性为0.0001 mg/cc。它似乎是一种粘蛋白。该抑制物在尿液中的活性与盐浓度呈负相关。
Normal human urine contains a highly active inhibitor of viral hemagglutination, effective against PR8, Lee, FM1, swine, mumps, Newcastle disease, and GDVII viruses,but inactive against PVM. Urine from cats, rabbits and guinea pigs also shows inhibitory activity. The inhibitor concns. are of the order of 2 mg. %. The inhibitor was isolated from human urine by means of 3 procedures: alcohol precipitation; precipitation at pH 3; and precipitation with 0.58 [image] NaCl. After further purification the material was active at 0.0001 mg./cc. It appears to be a mucoprotein. The activity of the inhibitor as it occurs in urine is inversely related to salt concn.