Delayed Deproteinization Causes Methodological Errors in Amino Acid Levels in Plasma Stored at Room Temperature or-20°C
Delayed Deproteinization Causes Methodological Errors in Amino Acid Levels in Plasma Stored at Room Temperature or-20°C
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DOI：
10.5713/ajas.2009.90156
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发表时间：
2009-12-01
期刊：
影响因子：
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通讯作者：
Manabe, Noboru
中科院分区：
文献类型：
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作者：
Li, Junyou;Piao, Chunxiang;Manabe, Noboru
脱蛋白被认为是血浆样品氨基酸分析的先决条件。对于室温下储存的血浆，延迟脱蛋白30、60或120分钟不会导致平均CV（变异系数）发生显著变化，其范围为4.4%至5.6%。然而，天冬氨酸、α-氨基己二酸、丙氨酸和赖氨酸的平均CV约为10%。当血浆在-20 ℃下冷冻储存时，解冻后0分钟和120分钟的CV分别增加至12.4%（范围：4.1 - 35.3%）和8.0%（2.5 - 30.7%）。在室温下储存期间，分析的所有氨基酸的血浆浓度均显示出显著变化。在室温下储存30分钟的血浆中，17种氨基酸浓度增加，2种氨基酸浓度降低。与参考组相比，将该时间段延长至60或120分钟增加了不稳定性。在-20 ℃下储存血浆2周导致氨基酸浓度的变化比室温下显著更大。在室温下，解冻后延长储存时间至30、60和120分钟，分别有21、20和所有22种氨基酸发生显著变化（p
Deproteinization has been recognized as a prerequisite for amino acid analysis of plasma samples. For plasma stored at room temperature, delaying deproteinization for 30, 60 or 120 minutes did not result in significant changes in the mean CV (coefficient of variation), which ranged from 4.4 to 5.6%. However the mean CV of aspartic acid, alpha-aminoadipic acid, alanine and lysine was about 10%. When the plasma was stored frozen at -20 degrees C, the CV was increased at 0 and 120 minutes after thawing, to 12.4% (range, 4.1 to 35.3%) and 8.0% (2.5 to 30.7%), respectively. The concentrations in plasma during storage at room temperature of all the amino acids analyzed showed significant changes. In plasma stored for 30 minutes at room temperature, 17 amino acids increased in concentrations and two decreased. Extending this period to 60 or 120 minutes increased the instability as compare to the reference group. Storing plasma at -20 degrees C for 2 weeks resulted in significantly greater changes in the amino acid concentrations than at room temperature. On extending the storage time at room temperature, after thawing, to 30, 60, and 120 minutes, 21, 20, and all 22 amino acids respectively changed significantly (p