Immunohistochemical analysis of cytokeratins in ameloblastoma and adenomatoid odontogenic tumor
Immunohistochemical analysis of cytokeratins in ameloblastoma and adenomatoid odontogenic tumor
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DOI：
10.1590/s1676-24442005000600009
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发表时间：
2005-12-01
影响因子：
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通讯作者：
Freitas, Roseana de Almeida
中科院分区：
文献类型：
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作者：
Lopes, Fernanda Ferreira;Cruz, Maria Carmen Fontoura Nogueira de;Freitas, Roseana de Almeida
目的：探讨细胞角蛋白(CKs)7、8、10、13、14、18和19在成釉细胞瘤和牙源性腺瘤(AOT)中的表达。对结果进行了比较，并讨论了组织发生。材料与方法：应用免疫组织化学链霉亲和素-生物素-过氧化物酶复合法和抗CKS抗体对10例成釉细胞瘤和8例牙源性腺瘤标本进行检测。样本来自巴西北里奥格兰德州联邦大学口腔病理学系。结果：CK14在所有成釉细胞瘤中均呈免疫组织化学阳性反应，CK19广泛表达于8例成釉细胞瘤外周细胞和5例成釉细胞瘤中央细胞。在AOTS中，CK14均呈阳性表达，而CK 19主要表达于导管细胞(6例)。结论：CKS在成釉细胞瘤和AOTS中以多种形式表达，表现为典型的牙胚CKS，提示这些肿瘤有牙源性上皮分化，而没有表现出正常的鳞状上皮CKS。
Objectives: The aim of the present study was to describe the immunohistochemical expression of cytokeratins (CKs) 7, 8, 10,13, 14, 18 and 19 in the epithelial components of ameloblastomas and adenomatoid odontogenic tumour (AOT). The results were compared and histogenesis discussed. Material end method: Specimens of ten ameloblastomas and eight adenomatoid odontogenic tumours were examined by immunohistochemistry using streptavidin-biotin-peroxidase complex method and anti-CKs antibody. The sample was obtained from Department of Oral Pathology, Federal University of Rio Grande do Norte, Brazil. Results: Immunohistochemical reactivity for CK14 was detected in all cases of ameloblastomas, and the expression of CK19 was diffusely present in peripheral cells (eight cases) and in central cells of five ameloblastomas. In AOTs, immunopositivity for CK14 was detected in all cases, whereas CK 19 was predominantly expressed in ductal cells (six cases). Conclusion: The CKs was expressed by several forms in ameloblastomas and AOTs, that have shown typical CKs of dental germ suggesting that these tumours have odontogenic epithelial differentiation, whereas it has not evinced proper CKs of squamous epithelial.