Bid-deficient fish delay grass carp reovirus (GCRV) replication and attenuate GCRV-triggered apoptosis.
Bid-deficient fish delay grass carp reovirus (GCRV) replication and attenuate GCRV-triggered apoptosis.
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DOI：
10.18632/oncotarget.19460
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发表时间：
2017-09-29
期刊：
影响因子：
--
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
He L;Wang H;Luo L;Li Y;Huang R;Liao L;Zhu Z;Wang Y
Bid， bh3相互作用结构域死亡激动剂，是Bcl-2家族中仅支持bh3的促凋亡分子，在细胞凋亡中起重要作用。本研究克隆并分析了草鱼（Ctenopharyngodon idellus）和稀有鲦鱼（Gobiocypris rarus）的Bid基因，分别命名为CiBid和GrBid。Bid在草鱼各组织中均有组成性表达，但在不同组织中的表达水平不同。草鱼呼肠孤病毒（GCRV）体内刺激后，Bid和凋亡相关基因Caspase-9和Caspase-3在感染后期显著上调。此外，我们制造了一个缺乏Bid的罕见小鱼（Bid-/-）来研究Bid在gcrv引发的细胞凋亡中的可能作用。我们发现，与野生型鱼相比，Bid-/- rare minow感染GCRV后存活时间延长，GCRV在Bid-/- rare minow中的相对拷贝数低于野生型鱼，Caspase-9和Caspase-3在Bid-/- rare minow中的表达水平显著低于野生型鱼。总的来说，目前的数据揭示了Bid在硬骨鱼病毒诱导的细胞凋亡过程中的重要作用。本研究为进一步了解GCRV诱导草鱼细胞凋亡提供了新的思路，并为草鱼抗病毒育种提供了靶点基因。
Bid, BH3-interacting domain death agonist, is a pro-apoptotic BH3-only member of Bcl-2 family, playing an important role in apoptosis. In the study, Bid genes from grass carp (Ctenopharyngodon idellus) and rare minnow (Gobiocypris rarus), named CiBid and GrBid, were cloned and analyzed. Bid was constitutively expressed in all examined tissues of grass carp, but the expression level varied in different tissues. Following grass carp reovirus (GCRV) stimulation in vivo, Bid and apoptosis related genes Caspase-9 and Caspase-3 was up-regulated significantly at the late stage of infection. Moreover, we generated a Bid-deficient rare minnow (Bid-/-) to investigate the possible role of Bid in GCRV-triggered apoptosis. We found that the survival time of Bid-/- rare minnow after GCRV infection was extended when compared with wild-type fish, the relative copy number of GCRV in Bid-/- rare minnow was lower than that in wild-type fish, and the expression level of Caspase-9 and Caspase-3 in Bid-/- rare minnow were significantly lower than that in the wild-type fish. Collectively, the current data revealed the important role of Bid during virus-induced apoptosis in teleost fish. Our study would provide new insight into understanding the GCRV induced apoptosis and may provide a target gene for virus-resistant breeding in grass carp.
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