Progesterone receptor A and B isoforms in the human breast and its disorders.
Progesterone receptor A and B isoforms in the human breast and its disorders.
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DOI：
10.1111/j.1349-7006.2001.tb01095.x
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发表时间：
2001-03
期刊：
影响因子：
--
通讯作者：
Sasano H
中科院分区：
文献类型：
--
作者：
Ariga N;Suzuki T;Moriya T;Kimura M;Inoue T;Ohuchi N;Sasano H
孕激素受体(PR)的两种不同亚型PRA和PRB在靶组织中的表达水平相当。在这项研究中，我们首先检测了人乳腺癌和各种导管内增生性上皮病变中PRA和PRB的免疫反应性，并将这些发现与临床病理参数相关联。然后，我们用RT-PCR方法检测了6例浸润性导管癌组织中PRA和PRB基因的表达。PRA和PRB在绝大多数非异型性增生性疾病(PDWA)(PRA和PRB分别为85%和96%)和不典型导管增生(ADH)(PRA和PRB分别为100%和100%)中均呈阳性表达，但在导管原位癌(DCIS)(PRA和PRB分别为65%和75%)和浸润性导管癌(IDC)(PRA和ADH分别为66%和55%)中免疫阳性例数和免疫组织化学(IHC)评分均显著低于PDWA和ADH。雌激素受体α(ERα)与雌激素受体α呈显著正相关(P<0.05)。在IDC中，PRA和PRB IHC评分均与组织学分级显著相关，但PRA或PRB状态与患者的淋巴转移、肿瘤大小或预后无关。在所有6例IDC中均检测到PRA和PRB的mRNA表达。这些结果表明，PRA和PRB在人类乳腺中都与ERA密切相关，这种关系在乳腺癌中可能受到干扰。
Two different isoforms of progesterone receptor (PR), PRA and PRB, are expressed in target tissues at comparable levels. In this study, we first examined PRA and PRB immunoreactivity in human breast cancer and various intraductal proliferative epithelial lesions, and correlated these findings with clinicopathologic parameters. We then examined mRNA expression of PRA and PRB in six cases of invasive ductal carcinoma using RT‐PCR. Immunoreactivity for both PRA and PRB was positive in the great majority of proliferative disease without atypia (PDWA) (85% for PRA and 96% for PRB) and atypical ductal hyperplasia (ADH) (100% for PRA and 100% for PRB), but the ratio of immunopositive cases and immunohistochemical (IHC) scores was significantly smaller in ductal carcinoma in situ (DCIS) (65% for PRA and 75% for PRB) and invasive ductal carcinoma (IDC) (66% for PRA and 55% for PRB) than in PDWA and ADH. There was a significant positive correlation between IHC scores for PRA and estrogen receptor α (ERα) in IDC, DCIS and ADH but not between PRB and ERα. In IDC, both PRA and PRB IHC scores were significantly associated with histological grade, but there was no association between PRA or PRB status and lymph node involvement, tumor size, or prognosis of the patients. The expression of mRNAs for both PRA and PRB was detected in all six cases of IDC examined. These results suggest that both PRA and PRB are strongly associated with ERa in human breast and this relation may be disturbed in breast cancer.
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影响因子：
11.4
作者：
KASTNER, P;KRUST, A;CHAMBON, P
通讯作者：
CHAMBON, P
影响因子：
--
作者：
TUNG, L;MOHAMED, MK;HORWITZ, KB
通讯作者：
HORWITZ, KB
影响因子：
45.3
作者：
Harvey, JM;Clark, GM;Allred, DC
通讯作者：
Allred, DC
影响因子：
4.8
作者：
CLARKE, CL;ZAINO, RJ;SATYASWAROOP, PG
通讯作者：
SATYASWAROOP, PG
影响因子：
8
作者：
Adriaenssens, E;Lottin, S;Curgy, JJ
通讯作者：
Curgy, JJ