The molecular architecture of lamins in somatic cells.
The molecular architecture of lamins in somatic cells.
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DOI：
10.1038/nature21382
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发表时间：
2017-03-09
期刊：
影响因子：
64.8
通讯作者：
Medalia O
中科院分区：
文献类型：
--
作者：
Turgay Y;Eibauer M;Goldman AE;Shimi T;Khayat M;Ben-Harush K;Dubrovsky-Gaupp A;Sapra KT;Goldman RD;Medalia O
核纤层是后生动物细胞核的基本组成部分。它主要由核纤层蛋白组成，核纤层蛋白是组装成丝状网络的中间丝蛋白，桥接核膜和染色质。除了提供核的结构稳定性外，纤层还参与许多核活动，包括染色质组织，转录和复制。然而，核板层的结构组织知之甚少。在这里，我们使用冷冻电子断层扫描（cryo-ET），以获得一个详细的看法，该组织的层网状层内。单个核纤层蛋白丝的数据分析解决了球形装饰的纤维外观，并显示A-和B-型核纤层蛋白组装成四聚体3.5 nm厚的丝。因此，核纤层蛋白表现出与其他典型细胞骨架元件显著不同的结构。我们的研究结果定义了分子分辨率的核纤层网的结构，提供了深入了解它们在核纤层支架中的作用。
The nuclear lamina is a fundamental constituent of metazoan nuclei. It is composed mainly of lamins, which are intermediate filament proteins that assemble into a filamentous meshwork, bridging the nuclear envelope and chromatin . Besides providing structural stability to the nucleus , the lamina is involved in many nuclear activities, including chromatin organization, transcription and replication . However, the structural organization of the nuclear lamina is poorly understood. Here, we use cryo-electron tomography (cryo-ET) to obtain a detailed view of the organization of the lamin meshwork within the lamina. Data analysis of individual lamin filaments resolves a globular-decorated fiber appearance and shows that A- and B-type lamins assemble into tetrameric 3.5 nm thick filaments. Thus, lamins exhibit a structure that is remarkably different from the other canonical cytoskeletal elements. Our findings define the architecture of the nuclear lamin meshworks at molecular resolution, providing insights into their role in scaffolding the nuclear lamina.
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影响因子：
4
作者：
Meister, Peter;Mango, Susan E.;Gasser, Susan M.
通讯作者：
Gasser, Susan M.
影响因子：
16.6
作者：
Eibauer, Matthias;Pellanda, Mauro;Turgay, Yagmur;Dubrovsky, Anna;Wild, Annik;Medalia, Ohad
通讯作者：
Medalia, Ohad
影响因子：
64.8
作者：
NOGALES, E;WOLF, SG;DOWNING, KH
通讯作者：
DOWNING, KH
影响因子：
--
作者：
JONES, JCR;GOLDMAN, AE;GOLDMAN, RD
通讯作者：
GOLDMAN, RD
DOI：
10.1073/pnas.83.17.6450
发表时间：
1986-09-01
影响因子：
11.1
作者：
FISHER, DZ;CHAUDHARY, N;BLOBEL, G
通讯作者：
BLOBEL, G