Inhibition of prohormone convertase 1 (PC1) expression in cholecystokinin (CCK) expressing At-T20 cells decreased cellular content and secretion of CCK and caused a shift in molecular forms of CCK secreted
Inhibition of prohormone convertase 1 (PC1) expression in cholecystokinin (CCK) expressing At-T20 cells decreased cellular content and secretion of CCK and caused a shift in molecular forms of CCK secreted
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DOI：
10.1016/j.peptides.2005.09.015
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发表时间：
2006-04-01
期刊：
影响因子：
3
通讯作者：
Marchand, JE
中科院分区：
文献类型：
--
作者：
Beinfeld, MC;Vishnuvardhan, D;Marchand, JE
采用两种不同的RNAi方法抑制At-T20细胞中激素原转化酶1（prohormone convertase 1，PC 1）的表达。双链RNA的瞬时转染和表达靶向PC 1的发夹环RNA的载体的稳定表达减少了At-T20细胞的胆囊收缩素（CCK）分泌。PC 1 mRNA和蛋白表达在转染细胞中也明显降低。这种治疗引起胆囊收缩素（CCK）分泌形式的转变，降低CCK 22和增加CCK 8。稳定表达的RNAi有效地降低了PC 1的表达。在这些RNAi处理中观察到的CCK减少进一步支持了PC 1在这些细胞中CCK加工中的作用。(c)2005年爱思唯尔公司All rights reserved.
Two different RNAi methods were used to inhibit the expression of prohormone convertase 1 (PC1) in At-T20 cells. Transient transfection of double stranded RNA and stable expression of a vector expressing hairpin-loop RNA targeting PC1 reduced cholecystokinin (CCK) secretion from At-T20 cells. PC1 mRNA and protein were also decreased in the vector transfected cells. This treatment caused a shift in the forms of cholecystokinin (CCK) secreted, decreasing CCK 22 and increasing CCK 8. Stable expression of RNAi effectively decreased PC1 expression. The observed decrease in CCK seen with these RNAi treatments further supports a role for PC1 in CCK processing in these cells. (c) 2005 Elsevier Inc. All rights reserved.