DOWN-REGULATION OF CLASS-I HLA ANTIGENS AND OF THE EPSTEIN-BARR VIRUS-ENCODED LATENT MEMBRANE-PROTEIN IN BURKITT-LYMPHOMA LINES
DOWN-REGULATION OF CLASS-I HLA ANTIGENS AND OF THE EPSTEIN-BARR VIRUS-ENCODED LATENT MEMBRANE-PROTEIN IN BURKITT-LYMPHOMA LINES
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DOI：
10.1073/pnas.84.13.4567
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发表时间：
1987-07-01
影响因子：
11.1
通讯作者：
KLEIN, G
中科院分区：
文献类型：
--
作者：
MASUCCI, MG;TORSTEINDOTTIR, S;KLEIN, G
Epstein-Barr virus (EBV)-carrying Burkitt lymphoma (BL) cells are relatively or completely resistant to the lytic effect of major histocompatibility complex class I HLA antigen-restricted cytotoxic T lmphocytes (CTLs)generated by stimulating lymphocytes of EBV-seropositive donors with the autologous EBV-transformed lymphoblastoid cell line (LCL). We previously found that EBV-negative and EBV-carrying BL lines derived from HLA-A11-positive donors were not only resistant to lysis by the HLA-A11-restricted CTL generated by stimulation with the autologous LCL, but also to HLA-A11-specific CTL derived from lymphocytes of an EBV-seronegative donor stimulated with an allogeneic LCL. Using the same and additional cell lines, we now show that the CTL resistance of the BL lines is probably due to a selective down-regulation of HLA-A11. We also show that the EBV-encoded latent membrane protein is expressed at a lower level in the EBV-carrying BL lines than in EBV-transformed LCLs. Only one of eight in vitro EBV-converted BL lines that shifted to a more LCL-like growth pattern expressed LMP at a high level. This line also reexpressed the HLA-A11 antigen that was undetectable in its EBV-negative progenitor. Our findings suggest that the typical BL cell phenotype is associated with low expression of both proteins.
