Characterisation of a novel lyssavirus isolated from Pteropid bats in Australia
Characterisation of a novel lyssavirus isolated from Pteropid bats in Australia
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DOI：
10.1016/s0168-1702(98)00025-2
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发表时间：
1998-04-01
期刊：
影响因子：
5
通讯作者：
Blacksell, SD
中科院分区：
文献类型：
--
作者：
Gould, AR;Hyatt, AD;Blacksell, SD
一种新的狂犬病病毒分离自澳大利亚的翼蝠（澳大利亚蝙蝠狂犬病病毒，ABLV）的特点是使用基因序列分析，电子显微镜和一组单克隆抗体。对翼蝠和小鼠脑材料以及从组织培养基中分离的病毒进行的电子显微镜检查显示存在弹状弹状病毒颗粒和狂犬病病毒的结构特征。使用核衣壳（N）特异性单克隆抗体的分析显示，这种新的狂犬病病毒与血清1型狂犬病之间存在很强的相关性。ABLV原型株的核苷酸序列从核衣壳蛋白（N蛋白）的起始甲硫氨酸密码子到聚合酶基因（L蛋白）的氨基末端测定，距离为5344个核苷酸。比较推导的N、磷蛋白（P）、基质蛋白（M）和糖蛋白（G）蛋白表明，ABLV与血清1型经典狂犬病病毒的关系比与狂犬病病毒属的其他成员更密切。当与PV（巴斯德疫苗株）狂犬病的同源蛋白相比时，ABLV蛋白的N、P、M和G蛋白的百分比相关性分别为92%、75%、87%和75%。N蛋白序列的系统发育研究清楚地表明，ABLV是一个未被认可的成员的Lyssavirus属，并代表了一个新的基因型，基因型7。(C)1998 Elsevier Science B.V.保留所有权利。
A novel lyssavirus isolated from Pteropid bats in Australia (Australian Bat Lyssavirus, ABLV) has been characterised using gene sequence analyses, electron microscopy and a panel of monoclonal antibodies. Electron microscopic examination of Pteropid bat and mouse brain material as well as virus isolated from tissue culture medium, showed the presence of bullet-shaped rhabdovirus particles and structures characteristic of lyssavirus. Analysis using nucleocapsid (N) specific monoclonal antibodies, showed a strong relationship between this new lyssavirus and serotype 1 rabies. The nucleotide sequence of the prototype strain of ABLV was determined from the initiator methionine codon for the nucleocapsid protein (N protein) to the amino terminus of the polymerase gene (L protein), a distance of 5344 nucleotides. Comparisons of the deduced N, phosphoprotein (P), matrix protein (M), and glycoprotein (G) proteins showed that ABLV was more closely related to serotype 1 classic rabies viruses than to other members of the Lyssavirus genus. The percent relatedness of the ABLV proteins when compared to the cognate proteins of PV (Pasteur vaccine strain) rabies was 92, 75, 87 and 75% for the N, P, M and G proteins, respectively. Phylogenetic studies of N protein sequences showed clearly that ABLV is an unrecognised member of the Lyssavirus genus and represents a new genotype, genotype 7. (C) 1998 Elsevier Science B.V. All rights reserved.