Determination of the spatial and temporal variability of phytoplankton community structure in Daya Bay via HPLC–CHEMTAX pigment analysis
Determination of the spatial and temporal variability of phytoplankton community structure in Daya Bay via HPLC–CHEMTAX pigment analysis
复制标题
HPLC-CHEMTAX色素分析测定大亚湾浮游植物群落结构的时空变异
DOI：
10.1007/s00343-018-7103-z
复制
发表时间：
--
影响因子：
--
通讯作者：
Tao Jiang
中科院分区：
文献类型：
--
作者：
Longhua Wang;Linjian Ou;Kaixuan Wang;Chao Chai;Zhaohui Wang;Xiaomin Wang;Tao Jiang
利用HPLC-CHEMTAX分析技术研究了大亚湾浮游植物群落结构的时空变化。叶绿素（Chla）含量夏季最高（平均值为0.84 μg/L），冬季最低（平均值为0.33 μg/L）。CHEMTAX处理揭示了大亚湾浮游植物的季节性演替。冬季浮游植物以硅藻为主，占叶绿素a总量的41.5%。甲藻在浮游植物总生物量中所占比例随温度和氮磷比的升高而显著增加，春季达52.2%。在夏季，养分限制从磷转移到氮。此外，这一时期与硅藻的优势，占71.1%的叶绿素a。Prasinophytes和cryptophytes是另外两个优势群体，特别是在冬季占主导地位。蓝藻在夏秋季节成为重要类群。典范对应分析表明，金藻，甲藻，隐藻与高硝酸盐浓度，铵，溶解无机氮（DIN），和N/P比，并与温度和磷酸盐呈负相关。硅藻和蓝藻强烈相关的温度，磷酸盐和盐度，硝酸盐，铵，DIN和N/P比的负面影响。硅藻和甲藻在海湾的显微镜观察和色素HPLC信息是在良好的协议。本研究证明了色素分析在调查大亚湾这样复杂的物理环境中浮游植物类群分布的有用性。
The spatial and temporal variability of the phytoplankton community structure in Daya Bay, South China Sea, were identified by using HPLC-CHEMTAX analytical techniques. The highest chlorophylla(Chla) concentrations were observed during summer (with an average value of 0.84 μg/L) and lowest ones during winter (with an average value of 0.33 μg/L). CHEMTAX processing revealed the seasonal succession of phytoplankton species in Daya Bay. During winter, diatoms were the dominant phytoplankton species and contributed 41.5% to total Chla. Based on Chlaconcentration, the average ratio of dinoflagellates to total phytoplankton biomass substantially increased with increasing temperature and nitrogen to phosphorus (N/P) ratio, reaching 52.2% in spring. Nutrient limitation shifted from phosphorus to nitrogen during summer. Moreover, this period was associated with the predominance of diatoms, which accounted for 71.1% of Chla. Prasinophytes and cryptophytes were the other two dominant groups and particularly dominated during winter. Cyanobacteria became an important group during summer and autumn. Canonical correspondence analysis suggested that chrysophytes, dinoflagellates, and cryptophytes were strongly associated with high nitrate concentration, ammonium, dissolved inorganic nitrogen (DIN), and N/P ratio, and were negatively associated with temperature and phosphate. Diatoms and cyanobacteria were strongly associated with temperature, phosphate, and salinity, and are negatively influenced by nitrate, ammonium, DIN, and N/P ratio. Microscopic observations and pigment HPLC information were in good agreement for diatoms and dinoflagellates in the bay. This study demonstrated the usefulness of pigment analysis in investigating the distribution of phytoplankton groups in a complex physical environment, such as Daya Bay.