POLYPEPTIDES IN SUCCINATE-COENZYME-Q REDUCTASE SEGMENT OF RESPIRATORY-CHAIN .8.
POLYPEPTIDES IN SUCCINATE-COENZYME-Q REDUCTASE SEGMENT OF RESPIRATORY-CHAIN .8.
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DOI：
10.1021/bi00645a009
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发表时间：
1977-01-01
期刊：
影响因子：
2.9
通讯作者：
MERLI, A
中科院分区：
文献类型：
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作者：
CAPALDI, RA;SWEETLAND, J;MERLI, A
用聚丙烯酰胺凝胶电泳将牛心线粒体复合物II（琥珀酸辅酶Q还原酶）拆分为10种不同的多肽。四种多肽，CII-1，CII-2，CII-3和CII-4，MW为70，000，24，000，13，500和7000，在所有检查的制剂中大量存在。CII-1和CII-2分别是琥珀酸脱氢酶的黄素蛋白和Fe-S蛋白; CII-3和CII-4尚未进行功能鉴定。六种多肽存在于更小的量，通过染色强度判断，和这些comigrated与复合物III中的组件。复合物II中的几个次要组分的氨基酸组成与复合物III中的等效迁移多肽的氨基酸组成相同。琥珀酸辅酶Q还原酶显然含有4种不同的多肽，并且当分离为复合物II时被不同量的复合物III污染。
Beef heart mitochondria complex II (succinate-coenzyme Q reductase) was resolved into 10 different polypeptides by polyacrylamide gel electrophoresis. Four polypeptides, CII-1, CII-2, CII-3 and CII-4 with MW of 70,000, 24,000, 13,500 and 7000, were present in large amounts in all preparations examined. CII-1 and CII-2 are the flavoprotein and Fe-S protein, respectively, of succinate dehydrogenase; CII-3 and CII-4 were not functionally identified. Six polypeptides were present in much smaller amounts as judged by staining intensity, and each of these comigrated with components in complex III. Amino acid compositions of several of the minor components in complex II were identical with that of an equivalently migrating polypeptide in complex III. Succinate-coenzyme Q reductase apparently contains 4 different polypeptides and is contaminated with variable amounts of complex III when isolated as complex II.