THE FLUORESCENCE OF ELASTIC FIBERS STAINED WITH EOSIN AND EXCITED BY VISIBLE LIGHT HUMAN ANIMAL
THE FLUORESCENCE OF ELASTIC FIBERS STAINED WITH EOSIN AND EXCITED BY VISIBLE LIGHT HUMAN ANIMAL
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DOI：
10.1007/bf01081407
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
GOLDSTEIN D J
中科院分区：
文献类型：
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作者：
GOLDSTEIN D J
任何波长的荧光，由显微镜标本发射与可见光激发，可以观察使用交叉偏振光和暗场聚光镜。在光谱的绿色部分，伊红的吸光度很高，因此，来自钨丝灯的可见光在激发这种染料的荧光方面非常有效。用血红素和伊红染色的常规切片中发现弹性纤维荧光相当强烈，而大多数其他伊红染色的结构荧光较少或根本没有荧光。尽管这种现象可能在常规组织学中被证明是有用的，但伊红染色弹性纤维相对选择性荧光的原因尚不清楚。然而，有可能的是，在大多数染色底物中，伊红的荧光在很大程度上被染料分子的聚集淬灭，但在相对致密（即不渗透）的弹性纤维中，这种荧光被抑制。
Fluorescent light of any wavelength, emitted by a microscopical specimen excited with visible light, can be observed using crossed polarizers and a dark-field condenser. The absorbance of Eosin is high in the green portion of the spectrum, so that visible light from a tungsten lamp is highly effective in exciting the fluorescence of this dye.Elastic fibres in routine sections stained with Haemalum and Eosin have been found to fluoresce rather strongly, while most other Eosin-stained structures fluoresce less or not at all. The reason for this relatively selective fluorescence of Eosin-stained elastic fibres is obscure, although the phenomenon may prove useful in routine histology. It is possible, however, that in most stained substrates the fluorescence of Eosin is largely quenched by aggregation of the dye molecules, but that this is inhibited inside the relatively dense (i.e.impermeable) elastic fibres.