Effect of phytochrome-mediated red light signaling on phosphorus uptake and accumulation in rice
Effect of phytochrome-mediated red light signaling on phosphorus uptake and accumulation in rice
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DOI：
10.1080/00380768.2020.1798205
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发表时间：
2020-08
影响因子：
2
通讯作者：
Yasuhito Sakuraba;S. Yanagisawa
中科院分区：
文献类型：
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作者：
Yasuhito Sakuraba;S. Yanagisawa
摘要磷是植物生长和生产力所必需的大量营养元素。植物以无机磷（Pi）的形式吸收磷;然而，在自然生态系统中，Pi的有效性经常是植物生长的严重限制因素。因此，植物已经进化出几种机制，例如Pi饥饿响应基因的表达，以适应Pi缺乏的条件。虽然我们最近报道，光敏色素（Phy）介导的红光信号通过增加模式植物拟南芥中这些基因的表达水平来促进Pi的吸收，但在农作物中是否存在类似的机制仍然是难以捉摸的。本研究分析了红光信号对水稻（Oryza sativa L.）使用osphyA和osphyB单突变体以及osphyA osphyB双突变体。与osphyA幼苗不同，osphyB幼苗表现出对Pi的吸收和Pi含量的降低。此外，野生型幼苗的红光照射显着促进磷的吸收，而蓝光或远红光照射没有。的osphyB突变体表现出降低的表达水平的几个Pi饥饿响应基因，包括Pi转运蛋白基因。此外，osphyB敲除突变体的这些表型在Pi缺乏条件下比在Pi充足条件下明显得多。此外，红光促进了其他植物幼苗对磷的吸收，包括花椰菜（Brassica oleracea L.）变种italica）和莴苣（Latuca sativa L.）。这些结果表明，OsPhyB介导的红光信号通过上调与磷饥饿反应相关基因的表达来促进水稻对磷的吸收，并且这种现象可能在许多植物中存在。
ABSTRACT Phosphorus (P) is a macronutrient essential for plant growth and productivity. Plants uptake P as inorganic phosphate (Pi); however, in the natural ecosystem, Pi availability is frequently a severe limiting factor for plant growth. Thus, plants have evolved several mechanisms, such as the expression of Pi starvation-responsive genes, to adapt to Pi deficient conditions. Although we recently reported that phytochrome (Phy)-mediated red light signaling promotes Pi uptake by increasing expression levels of such genes in the model plant Arabidopsis, it remains elusive whether a similar mechanism exists in agricultural crops. In the present study, we analyzed the effects of red light signaling on Pi uptake in rice (Oryza sativa L.) using osphyA and osphyB single mutants, and the osphyA osphyB double mutant. Unlike osphyA seedlings, osphyB seedlings showed a reduction in Pi uptake and Pi content. Furthermore, illumination of wild-type seedlings with red light significantly promoted Pi uptake, whereas illumination with blue or far-red light did not. The osphyB mutant showed reduced expression levels of several Pi starvation-responsive genes including Pi transporter genes. Additionally, these phenotypes of osphyB knockout mutants were much more evident under Pi deficient conditions than under Pi sufficient conditions. Moreover, red light promoted Pi uptake in seedlings of other plant species including broccoli (Brassica oleracea L. var. italica) and lettuce (Latuca sativa L.). These results suggest that OsPhyB-mediated red light signaling promotes Pi uptake in rice by up-regulating the expression of Pi starvation response-associated genes, and this phenomenon may be conserved in a wide range of plant species.