Rapid stress-induced elevations in corticotropin-releasing hormone mRNA in rat central amygdala nucleus and hypothalamic paraventricular nucleus: An in situ hybridization analysis
Rapid stress-induced elevations in corticotropin-releasing hormone mRNA in rat central amygdala nucleus and hypothalamic paraventricular nucleus: An in situ hybridization analysis
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DOI：
10.1016/s0006-8993(98)00032-8
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发表时间：
1998-03-30
期刊：
影响因子：
2.9
通讯作者：
Kalin, NH
中科院分区：
文献类型：
--
作者：
Hsu, DT;Chen, FL;Kalin, NH
含有促肾上腺皮质激素释放激素（CRH）的高密度神经细胞位于杏仁核中央核（CeA）和下丘脑室旁核（PVN）。这些大脑区域在激活对压力的自主神经、行为和内分泌反应方面起着重要作用。本研究旨在为应激对CeA和PVN CRH mRNA表达的急性影响提供必要的信息。大鼠受约束应激1小时，应激后间隔1小时收集脑。采用原位杂交技术检测CeA和PVN中CRH mRNA的表达。密度分析显示，急性约束应激导致PVN和鼻侧CeA区CRH mRNA水平显著升高。此外，胁迫后，吻侧CeA中包含高CRH mRNA信号的区域显著增加。结果明确表明，应激后CeA和PVN的CRH神经元CRH mRNA表达迅速增加。(C) 1998爱思唯尔科学有限公司
High densities of nerve cells containing corticotropin-releasing hormone (CRH) are located in the central nucleus of the amygdala (CeA) and paraventricular nucleus (PVN) of the hypothalamus. These brain regions play an important role in activating autonomic, behavioral, and endocrine responses to stress. This study was conducted to provide needed information concerning the acute effects of stress on CeA and PVN CRH mRNA expression. Rats were exposed to restraint stress for 1 h and brains collected after a 1-h post-stress interval. CRH mRNA expression occurring in the CeA and PVN was examined using in situ hybridization techniques. Densitometric analysis revealed that acute restraint stress produced significant increases in CRH mRNA levels in the PVN and in the rostral CeA region. In addition, the area in the rostral CeA encompassing high CRH mRNA signals increased significantly after stress. Results provide clear evidence that CRH neurons in the CeA and PVN exhibit rapid increases in CRH mRNA expression after exposure to stress. (C) 1998 Elsevier Science B.V.