Differential effects of light/dark recombinant human prolactin administration on the submaxillary lymph nodes and spleen activity of adult male mice.
Differential effects of light/dark recombinant human prolactin administration on the submaxillary lymph nodes and spleen activity of adult male mice.
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光/暗重组人催乳素给药对成年雄性小鼠颌下淋巴结和脾脏活动的不同影响。
DOI：
10.1159/000075321
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Bartke,A
中科院分区：
文献类型：
--
作者：
Esquifino,AI;Arce,A;Alvarez,MP;Chacon,F;Brown-Borg,H;Bartke,A
目的：在成年雄性小鼠的颌下淋巴结和脾脏中，研究细胞结构、CD 4+、CD 8+和双阳性（CD 4 +-CD 8+）淋巴细胞百分比、脂多糖（LPS）和刀豆球蛋白A（Con A）诱导的淋巴细胞增殖和自然杀伤（NK）活性的昼夜变化，以及定时给予重组人催乳素（h-PRL）对上述参数的影响。当在黑暗期间给药时，h-PRL诱导CD 4+双阳性（CD 4 +-CD 8+）淋巴细胞百分比的免疫抑制。与未处理的对照相比，Con A和LPS诱导的淋巴细胞增殖和NK活性。当h-PRL在光照期间给药时，细胞结构增加，并且与对照相比，h-PRL在Con A和LPS诱导的淋巴细胞增殖和NK活性中具有免疫抑制作用。此外，在对照颌下淋巴结的细胞结构，CD 8+，双阳性淋巴细胞，在刀豆蛋白A和LPS和NK活性的存在下的囊胚增殖的百分比不同时，比较黑暗与光明阶段。与对照组相比，当在黑暗期间施用时，h-PRL诱导双阳性（CD 4 +-CD 8+）淋巴细胞、Con A-和LPS-诱导的淋巴细胞增殖的百分比的免疫抑制。当h-催乳素是在光周期期间，没有影响，observed.Conclusions：这些结果表明存在差异的日/夜变化的细胞免疫反应取决于淋巴器官考虑。由于管理的h-PRL的差异调制的昼夜变化也观察到。
Objectives:Day/night variations in cellularity, percentage of CD4+, CD8+ and double-positive (CD4+–CD8+) lymphocytes, lipopolysaccharide (LPS)- and concanavalin A (Con A)-induced lymphocyte proliferation and natural killer (NK) activity, and the effect of timed administration of recombinant human prolactin (h-PRL) on the above-mentioned parameters were investigated in the submaxillary lymph nodes and spleen of adult male mice.Results:In controls, the percentage of CD4+, double-positive lymphocytes, LPS- or Con A-induced blastogenic proliferation and NK activity in the spleen differ during the dark phase as compared to the light phase. When administered during the dark period, h-PRL induced immunosuppresion in the percentage of CD4+, double-positive (CD4+–CD8+) lymphocytes. Con A- and LPS-induced lymphocyte proliferation and NK activity as compared to untreated controls. When h-PRL was administered during the light period, the cellularity increased, and h-PRL was immunosuppressive in Con A- and LPS-induced lymphcoyte proliferation and NK activity as compared to controls. Moreover, in control submaxillary lymph nodes the cellularity, percentage of CD8+, double-positive lymphocytes, blastogenic proliferation in the presence of Con A and LPS and NK activity differ when comparing the dark with the light phase. When administered during the dark period h-PRL induced immunosuppression in the percentage of double-positive (CD4+–CD8+) lymphocytes, Con A- and LPS-induced lymphocyte proliferation as compared to controls. When h-PRL is administered during the light period, no effects were observed.Conclusions:These results indicate the existence of differential day/night variations in the cellular immune response depending upon the lymphoid organ considered. Because of the administration of h-PRL a differential modulation of this circadian variation was also observed.