Molecular characterization of exosome-like vesicles from breast cancer cells.
Molecular characterization of exosome-like vesicles from breast cancer cells.
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DOI：
10.1186/1471-2407-14-44
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发表时间：
2014-01-27
期刊：
影响因子：
3.8
通讯作者：
Izadpanah R
中科院分区：
文献类型：
--
作者：
Kruger S;Abd Elmageed ZY;Hawke DH;Wörner PM;Jansen DA;Abdel-Mageed AB;Alt EU;Izadpanah R
由肿瘤细胞释放到细胞外介质中的膜囊泡具有携带包括蛋白质和微小RNA（miRNA）的致癌分子阵列的潜力。细胞外（外泌体样）囊泡在细胞间通讯中起主要作用。因此，外泌体样囊泡的蛋白质和miRNA的表征对于阐明细胞间信号传导以及鉴定疾病标志物是必要的。使用梯度离心从MCF-7和MDA-MB 231培养物中分离外来体样囊泡。使用液相色谱-质谱法（LC-MS/MS）对囊泡的蛋白质组学分析揭示了来自MCF-7细胞（MCF-Exo）的外泌体样囊泡与来自MDA-MB 231细胞（MDA-Exo）的外泌体样囊泡的不同蛋白质谱。蛋白质数据库检索已鉴定出MDA-Exo中的88种蛋白质和MCF-Exo中的59种蛋白质。分析表明，在所有蛋白中，有27个蛋白在两种外泌体样囊泡类型之间是共同的。此外，MDA-Exo含有更高量的基质金属蛋白酶，这可能与MDA-MB 231细胞的转移特性增强有关。此外，微阵列分析鉴定了两种囊泡之间的几种致癌miRNA。外泌体样囊泡中致癌因子的鉴定是重要的，因为这样的囊泡可以将信号传递到非恶性细胞，并且可能在肿瘤进展和转移中具有意义。
Membrane vesicles released by neoplastic cells into extracellular medium contain potential of carrying arrays of oncogenic molecules including proteins and microRNAs (miRNA). Extracellular (exosome-like) vesicles play a major role in cell-to-cell communication. Thus, the characterization of proteins and miRNAs of exosome-like vesicles is imperative in clarifying intercellular signaling as well as identifying disease markers. Exosome-like vesicles were isolated using gradient centrifugation from MCF-7 and MDA-MB 231 cultures. Proteomic profiling of vesicles using liquid chromatography-mass spectrometry (LC-MS/MS) revealed different protein profiles of exosome-like vesicles derived from MCF-7 cells (MCF-Exo) than those from MDA-MB 231 cells (MDA-Exo). The protein database search has identified 88 proteins in MDA-Exo and 59 proteins from MCF-Exo. Analysis showed that among all, 27 proteins were common between the two exosome-like vesicle types. Additionally, MDA-Exo contains a higher amount of matrix-metalloproteinases, which might be linked to the enhanced metastatic property of MDA-MB 231 cells. In addition, microarray analysis identified several oncogenic miRNA between the two types vesicles. Identification of the oncogenic factors in exosome-like vesicles is important since such vesicles could convey signals to non-malignant cells and could have an implication in tumor progression and metastasis.
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