The overlap between regeneration and fibrosis in injured skeletal muscle is regulated by phosphatidylinositol 3-kinase/Akt signaling pathway - A bioinformatic analysis based on lncRNA microarray
The overlap between regeneration and fibrosis in injured skeletal muscle is regulated by phosphatidylinositol 3-kinase/Akt signaling pathway - A bioinformatic analysis based on lncRNA microarray
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损伤骨骼肌再生与纤维化的重叠受磷脂酰肌醇3激酶/Akt信号通路调控——基于lncRNA微阵列的生物信息学分析
DOI：
10.1016/j.gene.2018.06.001
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发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Chen Jiwu
中科院分区：
文献类型：
--
作者：
Sun Yaying;Sun Xiaomeng;Liu Shaohua;Liu Lei;Chen Jiwu
骨骼肌损伤后会经历变性、成肌和纤维化的病理过程。越来越多的证据表明，在损伤后的早期，纤维化和肌形成阶段可能在损伤的骨骼肌内重叠。然而，这种重叠背后的机制仍不清楚。在这里，我们进行了一个lncRNA微阵列，以确定在小鼠肌肉挫伤后7天激活的途径。KEGG分析表明，磷脂酰肌醇3-激酶/Akt（PI 3 K/Akt）信号级联被预测为lncRNA激活。在PI 3 K/Akt信号转导中，lncRNA靶向的主要基因是层粘连蛋白、胶原5和胶原6的亚基，这些亚基参与了肌源性或纤维化过程。逆转录-聚合酶链反应分析和免疫组化染色进一步证实了计算机预测。这些结果表明，重叠可能与通过lncRNA调节激活的PI 3 K/Akt通路有关。
Injured skeletal muscle would go through a sequence of the pathological phases of degeneration, myogenesis and fibrosis. Growing evidence indicated that fibrotic and myogenic phases might overlap within the injured skeletal muscle in the early time after injury. However, the mechanism underlying this overlapping remains unclear. Here, we performed an lncRNA microarray to identify the activated pathways in mice muscle seven days after contusion. KEGG analysis indicated that phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signaling cascade was predicted to be activated by lncRNAs. The top genes targeted by lncRNAs in PI3K/Akt signaling were subunits of laminin, collagen 5, and collagen 6, which participated in either myogenic or fibrotic process. Reverse transcriptase–polymerase chain reaction analysis and immunohistochemical stain further confirmed the prediction in silico. These results suggested that the overlap might be related to an activated PI3K/Akt pathway by lncRNA regulation.