Cloning, sequencing and expression of a novel homeobox gene AxNox-1 from the Mexican axolotl.
Cloning, sequencing and expression of a novel homeobox gene AxNox-1 from the Mexican axolotl.
复制标题
墨西哥蝾螈新型同源盒基因 AxNox-1 的克隆、测序和表达。
DOI：
10.1016/s0378-1119(98)00304-7
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
Lemanski,LF
中科院分区：
文献类型：
--
作者：
Gaur,A;Dube,DK;Lemanski,LF
我们从18期蝾螈胚胎cDNA文库中克隆并测序了含有同源盒基因axox -1的cDNA，该文库与其他已知的同源盒基因只有中等水平的相似性。cDNA的核苷酸序列具有335个氨基酸的开放阅读框，除同源结构域外，还存在酸性结构域和富含脯氨酸的结构域。该基因的转录本在胚胎发育的第4阶段可检测到，因此，转录本很有可能是母系贡献的。axox -1的表达水平在12期达到最高水平，随后在25期下降到非常低的水平。axox -1的高水平转录本后来在幼嫩和蜕变的成年蝾螈的大脑中被发现。在测试的其他一些器官中也发现了少量的这种信息。对整个坐骑和切片的原位杂交研究表明，该基因在发育过程中主要在神经组织中表达。
We have cloned and sequenced a cDNA containing a homeobox gene, AxNox-1, from a stage 18 axolotl embryonic cDNA library which shows only moderate levels of similarity to other known homeobox genes. The nucleotide sequence of the cDNA has an open reading frame for 335 amino acids and besides the homeodomain, there is an acidic domain and a proline-rich domain present in the protein. The transcripts for this gene are detectable at stage 4 of embryonic development and, hence, there is a good possibility that the transcripts are maternally contributed. Expression levels for AxNox-1 reach maximum levels by stage 12 of development and thereafter decline to very low levels by stage 25. High levels of the transcript for AxNox-1 are later found in the brains of both neotenous and metamorphosing adult axolotls. Low amounts of the message are also found to be present in a number of other organs that were tested. In situ hybridization studies on whole mounts and sections suggest that this gene is expressed predominantly in neural tissue during development.