Spatially and functionally distinct Ca2+ stores in sarcoplasmic and endoplasmic reticulum
Spatially and functionally distinct Ca2+ stores in sarcoplasmic and endoplasmic reticulum
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DOI：
10.1126/science.275.5306.1643
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发表时间：
1997-03-14
期刊：
影响因子：
56.9
通讯作者：
Blaustein, MP
中科院分区：
文献类型：
--
作者：
Golovina, VA;Blaustein, MP
钙（Ca2+）储存在肌浆和内质网（S-ER）的组织尚不清楚。采用高分辨率成像方法研究了完整培养的星形胶质细胞和动脉肌细胞S-ER中钙的储存和释放动态。S-ER呈连续的管状网络；然而，S-ER中的钙储存被组织成小的、空间上不同的隔间，这些隔间作为离散单元发挥作用。环吡唑酸（S-ER膜上钙泵的抑制剂）和咖啡因或瑞诺定在不同的、空间上分开的隔室中卸载。在被生理激动剂激活的细胞中，钙储存的异质性也被揭示出来。这些结果表明，细胞可以产生空间和时间上不同的钙信号来控制个体钙依赖过程。
The organization of calcium (Ca2+) stores in the sarco plasmic and endoplasmic reticulum (S-ER) is poorly understood. The dynamics of the storage and release of calcium in the S-ER of intact, cultured astrocytes and arterial myocytes were studied with high-resolution imaging methods. The S-ER appeared to be a continuous tubular network; nevertheless, calcium stores in the S-ER were organized into small, spatially distinct compartments that functioned as discrete units. Cyclopiazonic acid (an inhibitor of the calcium pump in the S-ER membrane) and caffeine or ryanodine unloaded different, spatially separate compartments. Heterogeneity of calcium stores was also revealed in cells activated by physiological agonists. These results suggest that cells can generate spatially and temporally distinct calcium signals to control individual calcium-dependent processes.