TRANSPORT OF ANTIBIOTICS AND METABOLITE ANALOGS BY SYSTEMS UNDER CYCLIC-AMP CONTROL - POSITIVE SELECTION OF SALMONELLA-TYPHIMURIUM-CYA AND CRP MUTANTS
TRANSPORT OF ANTIBIOTICS AND METABOLITE ANALOGS BY SYSTEMS UNDER CYCLIC-AMP CONTROL - POSITIVE SELECTION OF SALMONELLA-TYPHIMURIUM-CYA AND CRP MUTANTS
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DOI：
10.1128/jb.133.1.149-157.1978
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发表时间：
1978-01-01
影响因子：
3.2
通讯作者：
AMES, BN
中科院分区：
文献类型：
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作者：
ALPER, MD;AMES, BN
鼠伤寒沙门氏菌环 (c)AMP 控制系统中的突变体（cya = 腺苷酸环化酶，crp = cAMP 受体蛋白）对 22 种抗生素（包括磷霉素、萘啶酸和链霉素）和正常细菌燃料/碳源的 29 种抑制类似物的生长抑制具有部分抗性。这种抗性被用作cya和crp突变体的有效正选择的基础。这些抗生素和类似物通过通常用于运输燃料/碳源的运输系统进入细菌，这是由于抗生素和所涉及的特定运输系统的天然底物之间的结构相似性而实现的。这些运输系统均受到 cAMP 的正向控制，并且 cAMP 充当燃料/碳剥夺的信号分子（警报素）。为 cAMP 控制的操纵子内的层次结构提供了证据。该方法对于分析抗生素转运系统和 cAMP 超级控制系统非常有用。
Mutants in the cyclic(c)AMP control system in S. typhimurium (cya = adenyl cyclase, crp = cAMP receptor protein) were partially resistant to growth inhibition by 22 antibiotics (including fosfomycin, nalidixic acid and streptomycin) and 29 inhibitory analogs of normal bacterial fuel/C sources. This resistance was used as the basis of an efficient positive selection of cya and crp mutants. These antibiotics and analogs enter the bacteria through transport systems normally used for transporting fuel/C sources and this is accomplished because of a structural similarity between the antibiotic and the natural substrate of the particular transport system involved. These transport systems are all under positive control by cAMP and cAMP acts as a signal molecule (alarmone) for fuel/C deprivation. Evidence is provided for a hierarchy within operons controlled by cAMP. The methodology is useful for analyzing both antibiotic transport systems and the cAMP super-control system.