Catalog-library approach for the rapid and sensitive structural elucidation of oligosaccharides
Catalog-library approach for the rapid and sensitive structural elucidation of oligosaccharides
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DOI：
10.1021/ac990095r
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发表时间：
1999-09-01
影响因子：
7.4
通讯作者：
Lebrilla, CB
中科院分区：
文献类型：
--
作者：
Tseng, K;Hedrick, JL;Lebrilla, CB
我们获得了寡糖组分几乎完整的结构阐明，包括皮摩尔水平的序列、连接，甚至立体化学。 “目录库”方法用于阐明寡糖混合物中次要成分的结构。从糖蛋白家族释放的寡糖通常由一小组有限的单糖组成。在这方面，许多寡糖种类类似于涉及组合文库的合成中发现的产物。文库中巨大的结构多样性是几乎无限组合的结果，其中甚至可以排列少量的单糖。幸运的是，不同寡糖之间存在结构相似性，因为不同化合物之间保留了特定的子结构基序。我们建议可以通过碰撞诱导解离质谱法来识别和表征子结构基序的目录，该目录是由一组已通过例如核磁共振在结构上完全阐明的已知化合物构建的，该目录由属于一组特定子结构基序的特征碎片模式组成，碰撞诱导解离用于确定这些基序的存在并重建不太丰富的成分的结构。
We obtained the nearly complete structural elucidation of oligosaccharide components, including sequence, linkage, and even stereochemistry in the picomolar levels. The "catalog-library" approach is used for elucidating the structures of minor components in a mixture of oligosaccharides. Oligosaccharides released from a family of glycoproteins are often composed of a small finite set of monosaccharides. In this regard, the numerous oligosaccharide species are analogous to the products found in syntheses involving combinatorial libraries. The great structural diversity in the library is the result of the nearly infinite combinations in which even a small number of monosaccharides can be arranged, Fortunately, structural similarities exist between different oligosaccharides, as specific substructural motifs are preserved among different compounds. We propose that a catalog of substructural motifs can be identified and characterized by collision-induced dissociation mass spectrometry, The catalog is constructed from a set of known compounds that have been fully structurally elucidated by, for example, nuclear magnetic resonance, The catalog consists of the characteristic fragmentation patterns belonging to a set of specific substructural motifs, Collision-induced dissociation is used to determine the presence of these motifs and reconstruct the structures of less abundant components.