Cloning and characterization of the antigenic membrane protein (Amp) gene and in situ detection of Amp from malformed flowers infected with Japanese hydrangea phyllody phytoplasma
Cloning and characterization of the antigenic membrane protein (Amp) gene and in situ detection of Amp from malformed flowers infected with Japanese hydrangea phyllody phytoplasma
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DOI：
10.1094/phyto-98-7-0769
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发表时间：
2008-07-01
期刊：
影响因子：
3.2
通讯作者：
Namba, S.
中科院分区：
文献类型：
--
作者：
Arashida, R.;Kakizawa, S.;Namba, S.
在日本各地发现的日本绣球花叶状病（JHP）对园艺产业造成经济损失。感染JHP植原体的日本八仙花表现出与花畸形有关的几种病害症状，如绿化、叶状和增殖。本研究克隆并鉴定了JHP植原体抗原性膜蛋白（antigenic membrane protein，JHP-AMP）基因同源物，在大肠杆菌中表达了JHP-AMP蛋白，并获得了抗JHP-AMP的抗体。抗JHP-ESP 1的抗体与抗洋葱黄化植原体ESP 1蛋白的抗体无交叉反应。这种血清学特异性可能是由于α-淀粉酶蛋白中亲水结构域的高度多样性。原位检测结果表明，JHP植原体定位于畸形花的韧皮部组织中。本研究表明，JHP-BMP 1蛋白确实是一种膜蛋白，在感染JHP植原体的绣球花中以可检测的水平表达。
A Japanese hydrangea phyllody (JHP) disease found throughout Japan causes economic damage to the horticultural industry. JHP phytoplasma-infected Japanese hydrangea plants show several disease symptoms involved in floral malformations, such as virescence, phyllody and proliferation. Here, we cloned and characterized the antigenic membrane protein (Amp) gene homolog from the JHP phytoplasma (JHP-amp), expressed the JHP-Amp protein in Escherichia coli cells, and then obtained an antibody against JHP-Amp. The antibody against JHP-Amp had no cross-reactions with the antibody against the Amp protein from a closely related onion yellows phytoplasma. This serologic specificity is probably due to the high diversity of the hydrophilic domains in the Amp proteins. The in situ detection of the JHP-Amp protein revealed that the JHP phytoplasma was localized to the phloem tissues in the malformed flower. This study shows that the JHP-Amp protein is indeed a membrane protein, which is expressed at detectable level in the JHP phytoplasma-infected hydrangea.