Effects of intraduodenal injection of Lactobacillus johnsonii La1 on renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats
Effects of intraduodenal injection of Lactobacillus johnsonii La1 on renal sympathetic nerve activity and blood pressure in urethane-anesthetized rats
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DOI：
10.1016/j.neulet.2005.07.036
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发表时间：
2005-12-02
影响因子：
2.5
通讯作者：
Nagai, K
中科院分区：
文献类型：
--
作者：
Tanida, M;Yamano, T;Nagai, K
此前，有研究表明，用乳酸菌发酵的牛奶可以降低血压，这表明细菌的代谢物或成分具有降血压作用。为了检查一种乳酸杆菌——约氏乳杆菌 La1 (L/La1)（一种粘附在肠上皮细胞上的益生菌菌株）或其代谢物是否具有降血压作用，以及作用机制，我们确定了十二指肠内注射 L/La1 对乌拉坦麻醉大鼠血压 (BP) 和自主神经活动的影响。十二指肠内注射 LJLa1 可降低肾交感神经活动 (RSNA) 和血压，并增强胃迷走神经活动 (GVNA)。用组胺能 H3 受体拮抗剂硫哌丁胺进行预处理，消除了 LJLa1 对 RSNA、GVNA 和 BR 的影响。此外，下丘脑视交叉上核（SCN）（主要昼夜节律振荡器）的双侧损伤消除了 L/La1 引起的 RSNA 和 BP 抑制以及 GVNA 升高。这些发现表明，L/La1 或其代谢物可能通过改变大鼠中枢组胺能神经和视交叉上核的自主神经传递来降低血压。 (C) 2005 Elsevier Ireland Ltd. 保留所有权利。
Previously, it was shown that milk fermented with lactic acid bacteria lowers blood pressure, suggesting that metabolites or components of the bacteria have hypotensive action. To examine whether one of lactobacilli, Lactobacillus johnsonii La1 (L/La1), a probiotic strain adhesive onto intestinal epithelial cells, or its metabolite has hypotensive action, and if so the mechanism of action, we determined the effects of intraduodenal injection of L/La1 on blood pressure (BP) and the activity of autonomic nerves in urethane-anesthetized rats. Intraduodenal injection of LJLa1 reduced renal sympathetic nerve activity (RSNA) and BP and enhanced gastric vagal nerve activity (GVNA). Pre-treatment with thioperamide, a histaminergic H3-receptor antagonist, eliminated the effects of LJLa1 on RSNA, GVNA, and BR Furthermore, bilateral lesions of the hypothalamic suprachiasmatic nucleus (SCN), the master circadian oscillator, abolished the suppression of RSNA and BP and the elevation of GVNA caused by L/La1. These findings suggest that L/La1 or its metabolites might lower BP by changing autonomic neurotransmission via the central histaminergic nerves and the suprachiasmatic nucleus in rats. (C) 2005 Elsevier Ireland Ltd. All rights reserved.