Comparative Sequence Analysis of the Non-Protein-Coding Mitochondrial DNA of Inbred Rat Strains
Comparative Sequence Analysis of the Non-Protein-Coding Mitochondrial DNA of Inbred Rat Strains
复制标题
DOI：
10.1371/journal.pone.0008148
复制
发表时间：
2009-12-07
期刊：
影响因子：
3.7
通讯作者：
Luthman, Holger
中科院分区：
文献类型：
--
作者：
Abhyankar, Avinash;Park, Hee-Bok;Luthman, Holger
哺乳动物线粒体的正常功能需要核基因和线粒体基因的协调表达，这很可能是由于核基因组和线粒体基因组的共同进化所致。线粒体 DNA (mtDNA) 的非蛋白质编码区（包括 D 环、tRNA 和 rRNA 基因）构成了该调节表达单位的主要组成部分。在这里，我们对 27 个褐家鼠 mtDNA 序列的非蛋白质编码区进行了比较分析。 12S rRNA 有 2 个可变位置，16S rRNA 有 20 个可变位置，tRNA 基因中有 8 个可变位置，D 环中有 13 个可变位置。使用的三项中性测试中只有一项显示出 16S rRNA 和 tRNA-Cys 选择的统计显着证据。基于我们对保守序列的分析，我们提出在 16S rRNA 和 tRNA-Cys 中鉴定的一些可变核苷酸位置以及 D 环可能对线粒体功能及其调节很重要。
The proper function of mammalian mitochondria necessitates a coordinated expression of both nuclear and mitochondrial genes, most likely due to the co-evolution of nuclear and mitochondrial genomes. The non-protein coding regions of mitochondrial DNA (mtDNA) including the D-loop, tRNA and rRNA genes form a major component of this regulated expression unit. Here we present comparative analyses of the non-protein-coding regions from 27 Rattus norvegicus mtDNA sequences. There were two variable positions in 12S rRNA, 20 in 16S rRNA, eight within the tRNA genes and 13 in the D-loop. Only one of the three neutrality tests used demonstrated statistically significant evidence for selection in 16S rRNA and tRNA-Cys. Based on our analyses of conserved sequences, we propose that some of the variable nucleotide positions identified in 16S rRNA and tRNA-Cys, and the D-loop might be important for mitochondrial function and its regulation.