Biophysical studies on the differentiation of human CD14+ mono cytes into dendritic cells
Biophysical studies on the differentiation of human CD14+ mono cytes into dendritic cells
复制标题
DOI：
10.1385/cbb:45:1:19
复制
发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Chien, S
中科院分区：
文献类型：
--
作者：
Zeng, Z;Liu, X;Chien, S
树突状细胞（Dendritic cells，DCs）是目前已知的最有效的抗原呈递细胞（antigen presenting cells，APC），可在体外由CD 14（+）单核细胞（DC precursor cells）分化而来。未成熟DC主动摄取抗原和病原体，产生主要组织相容性复合物-肽复合物，并从抗原获得部位迁移到次级淋巴器官，成为与T淋巴细胞相互作用并刺激T淋巴细胞的成熟树突状细胞。在这个过程中，细胞必须经历变形才能穿过几个屏障，包括血管壁中的基底膜和间质结缔组织。为了进一步了解免疫反应激活和从外周组织向次级淋巴器官迁移的机制，我们应用生物物理和微观流变学方法研究了DC的体外发育过程。结果表明，不同发育阶段的DC在膜流动性、渗透脆性、膜粘弹性、红外光谱和细胞骨架结构等方面存在显著差异。
Dendritic cells (DCs), which are the most efficient antigen-presenting cells (APCs) currently known, can be derived from CD14(+) monocytes (DC predecessor cells) in vitro. Immature DCs actively take up antigens and pathogens, generate major histocompatability complex-peptide complexes, and migrate from the sites of antigen acquisition to secondary lymphoid organs to become mature dendritic cells that interact with and stimulate T-lymphocytes. During this process, the cells must undergo deformation to translocate through several barriers, including the basement membrane and interstitial connective tissue in the blood vessel wall. To further understand the mechanisms of the activation of immunological responses and the migration from peripheral tissue to secondary lymphoid organs, we have applied biophysical and microrheological methods to study the development processes of DCs in vitro. The results showed that membrane fluidity, osmotic fragility, membrane viscoelastic properties, infrared spectroscopy, and cytoskeleton organization of DCs exhibit significant differences in different developmental stages.