Intracellular Acoustic Droplet Vaporization in a Single Peritoneal Macrophage for Drug Delivery Applications
Intracellular Acoustic Droplet Vaporization in a Single Peritoneal Macrophage for Drug Delivery Applications
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DOI：
10.1021/la203212p
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发表时间：
2011-11-01
期刊：
影响因子：
3.9
通讯作者：
Yeh, Chih-Kuang
中科院分区：
文献类型：
--
作者：
Kang, Shih-Tsung;Yeh, Chih-Kuang
研究了单液滴负载的巨噬细胞(DLMS)中全氟戊烷(PFP)液滴的声学液滴汽化(ADV)。用同步高速摄影和空化检测观察单个DLm内细胞内ADV的瞬时响应。进一步应用超声B型成像，显示了一组DLMS的ADV生成的气泡的对比增强。结合在DLm中的PFP液滴在蒸发成气泡后可以从细胞体中释放出来。在气泡出现的同时，可以同时诱发惯性空化。气化术开始时出现的气泡合并可能有助于气体栓塞术和超声成像。巨噬细胞可以作为潜在的载体，将PFP液滴运送到肿瘤的无血管和缺氧区，用于超声控制的药物释放和基于ADV的肿瘤治疗。
This study investigated the acoustic droplet vaporization (ADV) of perfluoropentane (PFP) droplets in single droplet-loaded macrophages (DLMs) by insonation with single three-cycle ultrasound pulses. Transient responses of intracellular ADV within a single DLM were observed with synchronous high-speed photography and cavitation detection. Ultrasound B-mode imaging was further applied to demonstrate the contrast enhancement of ADV-generated bubbles from a group of DLMs. The PFP droplets incorporated in a DLM can be liberated from the cell body after being vaporized into gas bubbles. Inertial cavitation can be simultaneously induced at the same that bubbles appear. The coalescence of bubbles occurring at the onset of vaporization may facilitate gas embolotherapy and ultrasound imaging. Macrophages can be potential carriers transporting PFP droplets to avascular and hypoxic regions in tumors for ultrasound-controlled drug release and ADV-based tumor therapies.