The formation of reactive oxygen species catalyzed by neutral, aqueous extracts of NIST ambient particulate matter and diesel engine particles
The formation of reactive oxygen species catalyzed by neutral, aqueous extracts of NIST ambient particulate matter and diesel engine particles
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NIST 环境颗粒物和柴油发动机颗粒的中性水提取物催化的活性氧的形成
DOI：
10.1080/10473289.2000.10464231
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发表时间：
2000-11-01
影响因子：
2.7
通讯作者：
Aust, AE
中科院分区：
文献类型：
--
作者：
Ball, JC;Straccia, AM;Aust, AE
必须确定颗粒物的化学特性，以便了解易感人群吸入的低剂量颗粒物可能对人类健康产生何种影响。测定了两种环境颗粒样品（美国国家标准与技术研究所（NIST）标准参考物质（SRM）1648和1649）和两种柴油机颗粒样品（NIST SRM 1650和SRM 2975）的中性水提取物催化的活性氧物种的形成。通过测量在抗坏血酸存在下2-脱氧核糖形成的丙二醛来估计活性氧物质的形成;本试验中未加入H2 O2。SRM 1649，从华盛顿，DC收集的环境颗粒物，产生最多的丙二醛，而SRM 2975，从叉车收集的柴油颗粒物，产生最少的量。去铁胺抑制微粒样品中丙二醛的形成，提供了额外的数据来支持过渡金属参与活性氧物质生成的观察结果。在用于颗粒样品的相同条件下测定六种过渡金属硫酸盐（硫酸铁、硫酸铜、硫酸氧钒、硫酸钴、硫酸镍和硫酸锌）产生活性氧物质的能力，以便于颗粒和这些过渡金属之间的比较。使用离子耦合等离子体质谱法（ICP-MS）分析测量这些颗粒样品的水浸提液中过渡金属的浓度。Fe、Cu和V的浓度与这些颗粒样品提取物中产生的丙二醛的量之间存在定性一致性。这些数据表明，过渡金属可以从中性水溶液中的颗粒中溶解，并且这些金属能够催化活性氧物质的形成。
It is important to characterize the chemical properties of particulate matter in order to understand how low doses, inhaled by a susceptible population, might cause human health effects. The formation of reactive oxygen species catalyzed by neutral, aqueous extracts of two ambient particulate samples, National Institute of Standards & Technology (NIST) Standard Reference Materials (SRM) 1648 and 1649, and two diesel particulate samples, NIST SRM 1650 and SRM 2975, were measured. The formation of reactive oxygen species was estimated by measuring the formation of malondialdehyde from 2-deoxyribose in the presence of ascorbic acid; H2O2 was not added to this assay. SRM 1649, ambient particulate matter collected from Washington, DC, generated the most malondialdehyde, while SRM 2975, diesel particulate matter collected from a forklift, yielded the least amount. Desferrioxamine inhibited the formation of malondialdehyde from the particulate samples providing additional data to support the observation that transition metals were involved in the generation of reactive oxygen species. Six transition metal sulfates (iron sulfate, copper sulfate, vanadyl sulfate, cobalt sulfate, nickel sulfate, and zinc sulfate) were assayed for their ability to generate reactive oxygen species under the same conditions used for the particulate samples in order to facilitate comparisons between particles and these transition metals. The concentration of transition metals was measured in aqueous extracts of these particulate samples using ion-coupled plasma mass spectrometry (ICP-MS) analysis. There was qualitative agreement between the concentrations of Fe, Cu, and V and the amount of malondialdehyde produced from extracts of these particulate samples. These data suggest that transition metals can be dissolved from particles in neutral, aqueous solutions and that these metals are capable of catalyzing the formation of reactive oxygen species.