Visualizing in vivo liposomal drug delivery in real-time.
Visualizing in vivo liposomal drug delivery in real-time.
复制标题
实时可视化体内脂质体药物输送。
DOI：
10.1080/10611860701538651
复制
发表时间：
2007
影响因子：
4.5
通讯作者：
Helms,JillA
中科院分区：
文献类型：
--
作者：
Kim,Jae-Beom;Leucht,Philipp;Morrell,NathanT;Schwettman,HAlan;Helms,JillA
脂质体在有效小分子递送方面具有巨大潜力。然而，之前的研究因无法监控其内容的分发和发布而受到阻碍。在这里，作者展示了使用荧光素作为模型实时监测小分子递送。为了监测体内荧光素的释放，将荧光素包装在热敏脂质体中并递送至组成型表达荧光素酶的转基因小鼠中。他们的实验显示了脂质体内容物的实时热诱导释放。此外，该模型提供的证据表明热敏脂质体在很长一段时间内（〜3周）是稳定的，并且在加热时仍然释放其内容物。这些数据提出了一种用荧光素监测体内脂质体药物递送的策略。
Liposomes have tremendous potential for efficient small molecule delivery. Previous studies, however, have been hampered by an inability to monitor their distribution and release of contents. Here, the authors demonstrate the real time monitoring of small molecule delivery using luciferin as a model. To monitor the release of luciferinin vivo, luciferin was packaged in thermosensitive liposomes and delivered into transgenic mice that constitutively express luciferase. Their experiments show the thermally induced release of the liposomal content in real time. In addition, the model provides evidence that the thermosensitive liposomes are stable over a long period of time (∼3 weeks), and still release their content upon heating. These data present a strategy to monitor liposomal drug deliveryin vivowith luciferin.