GENOMIC ORGANIZATION AND EXPRESSION OF THE 3' END OF THE CANINE AND FELINE ENTERIC CORONAVIRUSES
GENOMIC ORGANIZATION AND EXPRESSION OF THE 3' END OF THE CANINE AND FELINE ENTERIC CORONAVIRUSES
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DOI：
10.1016/0042-6822(92)90174-n
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发表时间：
1992-11-01
期刊：
影响因子：
3.7
通讯作者：
ROTTIER, PJM
中科院分区：
文献类型：
--
作者：
VENNEMA, H;ROSSEN, JWA;ROTTIER, PJM
对犬冠状病毒（CCV）和猫肠道冠状病毒（FECV）3′端的基因组结构进行了分析，并与猫传染性腹膜炎病毒（FIPV）和猪传染性胃肠炎病毒（TGEV）的基因组结构进行了比较。后两者的比较先前揭示了FIPV基因组3 '端的额外开放阅读框（ORF），其在TGEV中缺失，目前被指定为ORF 6b。CCV和FECV基因组的3′端均含有6b相关的开放阅读框。在该抗原簇中的四种病毒中有三种存在ORF 6b，这强烈表明TGEV已通过缺失而丢失了该ORF。CCV ORF 6b与FIPV ORF 6b共线，但氨基酸序列的同源性仅为58%。与FIPV相比，FECV ORF 6b包含大的缺失，共线部分减少到60%。CCV和TGEV之间以及FECV和FIPV之间的同源性最高。先前，我们表明FIPV ORF 6b的表达产物可以通过免疫沉淀在感染的细胞中检测到（Vennemaet al.，1992年）。在本研究中，我们进行了类似的实验与CCV和FECV。在感染的细胞中，两种病毒都产生与FIPV 6b蛋白相关但不同的蛋白。
The genomic organization at the 3′ end of canine coronavirus (CCV) and feline enteric coronavirus (FECV) was determined by sequence analysis and compared to that of feline infectious peritonitis virus (FIPV) and transmissible gastroenteritis virus (TGEV) of swine. Comparison of the latter two has previously revealed an extra open reading frame (ORF) at the 3′ end of the FIPV genome, lacking in TGEV, which is currently designated ORF 6b. Both CCV and FECV possess 6b-related ORFs at the 3′ ends of their genomes. The presence of ORF 6b in three of four viruses in this antigenic cluster strongly suggests that TGEV has lost this ORF by deletion. The CCV ORF 6b is collinear with that of FIPV, but the predicted amino acid sequences are only 58% identical. The FECV ORF 6b contains a large deletion compared to that of FIPV, reducing the collinear part to 60%. The sequence homologies were highest between CCV and TGEV on the one hand and between FECV and FIPV on the other. Previously, we showed that the expression product of the FIPV ORF 6b can be detected in infected cells by immunoprecipitation (Vennemaet al., 1992). In the present study we have performed similar experiments with CCV and FECV. In infected cells both viruses produced proteins related to but different from the FIPV 6b protein.