The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations
The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations
复制标题
DOI：
10.1038/nature10355
复制
发表时间：
2011-08-25
期刊：
影响因子：
64.8
通讯作者：
Hoffmann, A. A.
中科院分区：
文献类型：
--
作者：
Walker, T.;Johnson, P. H.;Hoffmann, A. A.
登革热是人类最重要的蚊媒病毒性疾病，估计每年在 100 多个国家发生超过 5000 万例病例(1,2)。令人不安的是，登革热的地理范围目前正在扩大，疫情的严重程度也在增加(2-4)。登革热的控制方案非常有限，目前的重点是减少主要蚊媒埃及伊蚊的种群丰度(5,6)。这些策略未能减少热带社区的登革热发病率，迫切需要有效的替代方案。有人提出，昆虫的内共生细菌沃尔巴克氏体感染可用于控制登革热的新策略(7-9)。例如，wMelPop-CLA Wolbachia 菌株会缩短稳定转染品系中成年埃及伊蚊的寿命 (8)。预计这种缩短寿命的表型会降低登革热传播的可能性。最近发现，包括 wMelPop-CLA 在内的几种沃尔巴克氏体感染也可以直接影响昆虫对一系列昆虫和人类病原体感染的易感性(9-11)，这显着改变了沃尔巴克氏体感染控制人类疾病的潜力。在这里，我们描述了用无毒力的沃尔巴克氏体 wMel 菌株成功转染埃及伊蚊，该菌株以最小的表观适应性成本和高母体传播诱导生殖表型细胞质不相容，为入侵提供了最佳表型效应。在半田条件下，wMel 菌株在几代内从初始起始频率 0.65 增加到接近固定，相对于 wMelPop-CLA 菌株的试验，以更快的速度入侵埃及伊蚊种群。我们还表明，wMel 和 wMelPop-CLA 菌株可阻止埃及伊蚊中登革热血清型 2 (DENV-2) 的传播，从而为抑制登革热的实用方法奠定了基础 (12)。
Dengue fever is the most important mosquito-borne viral disease of humans with more than 50 million cases estimated annually in more than 100 countries(1,2). Disturbingly, the geographic range of dengue is currently expanding and the severity of outbreaks is increasing(2-4). Control options for dengue are very limited and currently focus on reducing population abundance of the major mosquito vector, Aedes aegypti(5,6). These strategies are failing to reduce dengue incidence in tropical communities and there is an urgent need for effective alternatives. It has been proposed that endosymbiotic bacterial Wolbachia infections of insects might be used in novel strategies for dengue control(7-9). For example, the wMelPop-CLA Wolbachia strain reduces the lifespan of adult A. aegypti mosquitoes in stably transinfected lines(8). This life-shortening phenotype was predicted to reduce the potential for dengue transmission. The recent discovery that several Wolbachia infections, including wMelPop-CLA, can also directly influence the susceptibility of insects to infection with a range of insect and human pathogens(9-11) has markedly changed the potential for Wolbachia infections to control human diseases. Here we describe the successful transinfection of A. aegypti with the avirulent wMel strain of Wolbachia, which induces the reproductive phenotype cytoplasmic incompatibility with minimal apparent fitness costs and high maternal transmission, providing optimal phenotypic effects for invasion. Under semi-field conditions, the wMel strain increased from an initial starting frequency of 0.65 to near fixation within a few generations, invading A. aegypti populations at an accelerated rate relative to trials with the wMelPop-CLA strain. We also show that wMel and wMelPop-CLA strains block transmission of dengue serotype 2 (DENV-2) in A. aegypti, forming the basis of a practical approach to dengue suppression(12).