Cell-to-cell binding induced by different lectins.
Cell-to-cell binding induced by different lectins.
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由不同凝集素诱导的细胞间结合。
DOI：
10.1083/jcb.65.2.247
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发表时间：
1975-05
期刊：
影响因子：
--
通讯作者：
Sachs L
中科院分区：
文献类型：
--
作者：
Rutishauser U;Sachs L
通过测定单细胞与固定在尼龙纤维上的凝集素包被细胞结合的能力，分析了伴刀豆球蛋白A（Con A）和来自小麦胚芽、大豆和蜡豆的凝集素诱导的细胞-细胞结合。使用的细胞是淋巴瘤、髓性白血病和正常成纤维细胞。与所有凝集素，细胞与细胞的结合被抑制，如果两个细胞用戊二醛预固定。然而，在大多数情况下，细胞与细胞的结合增强时，只有凝集素包被的细胞是prefixed。与正常的成纤维细胞，用胰蛋白酶的一个或两个细胞的治疗增强了由刀豆蛋白A和小麦胚芽凝集素诱导的细胞与细胞的结合。增加大豆凝集素受体数量的神经氨酸酶，只有在两种细胞都被处理的情况下才能增加细胞与细胞的结合。大豆凝集素和麦胚凝集素诱导的细胞间结合可被适当的竞争性糖抑制剂部分逆转，但不能逆转Con A诱导的结合。实验表明，凝集素诱导的细胞与细胞结合可通过凝集素受体密度不足、受体迁移率不足或诱导的受体聚集来阻止。这些效应可以解释在不同细胞类型和凝集素中观察到的细胞间结合和凝集的差异。他们还表明，不同的凝集素与各种细胞类型诱导的细胞与细胞的结合是由一种机制启动的，该机制涉及碰撞细胞上互补受体的对齐，以形成多个细胞-凝集素-细胞桥。
The cell-to-cell binding induced by concanavalin A (Con A) and the lectins from wheatgerm, soybean, and waxbean has been analyzed by measuring the ability of single cells to bind to lectin-coated cells immobilized on nylon fibers. The cells used were lymphoma, myeloid leukemia, and normal fibroblast cells. With all lectins, cell-to-cell binding was inhibited if both cells were prefixed with glutaraldehyde. However, in most cases cell-to-cell binding was enhanced when only the lectin-coated cell was prefixed. With normal fibroblasts, treatment of either one or both cells with trypsin enhanced the cell-to-cell binding induced by Con A and the wheatgerm lectin. Neuraminidase, which increases the number of receptors for soybean agglutinin, increased cell-to-cell binding only if both cells were treated. Although cell-to- cell binding induced by the lectins from soybean and wheatgerm could be partially reversed by the appropriate competitive saccharide inhibitor, binding induced by Con A could not be reversed. The experiments indicate that cell-to-cell binding induced by a lectin can be prevented by an insufficient density of receptors for the lectin, insufficient receptor mobility, or induced clustering of receptors. These effects can explain the differences in cell-to-cell binding and agglutination observed with different cell types and lectins. They also suggest that cell-to-cell binding induced by different lectins with a variety of cell types is initiated by a mechanism involving the alignment of complementary receptors on the colliding cells for the formation of multiple cell-to-lectin-to-cell bridges.