Proteomic and Transcriptomic Analyses of Rigid and Membranous Cuticles and Epidermis from the Elytra and Hindwings of the Red Flour Beetle, Tribolium castaneum
Proteomic and Transcriptomic Analyses of Rigid and Membranous Cuticles and Epidermis from the Elytra and Hindwings of the Red Flour Beetle, Tribolium castaneum
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DOI：
10.1021/pr2009803
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发表时间：
2012-01-01
影响因子：
4.4
通讯作者：
Kanost, Michael R.
中科院分区：
文献类型：
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作者：
Dittmer, Neal T.;Hiromasa, Yasuaki;Kanost, Michael R.
昆虫表皮是一种主要由甲壳素和蛋白质组成的复合生物材料。角质层的物理性质可以从硬和刚性到软和柔性变化很大。了解不同类型的角质层是如何组装的，可以帮助开发用于医学和技术的新型仿生材料。为了实现这一目标，我们已经采取了结合蛋白质组学和转录组学的方法与红粉甲虫，赤拟谷盗，检查蛋白质和基因表达谱的鞘翅和后翅，附件，包含刚性和软cupeles，分别。双向凝胶电泳分析显示，在蛋白质分布之间的鞘翅和后翅，与四个高度丰富的蛋白质占主导地位的鞘翅角质层提取物的明显差异。MALDI/TOF质谱鉴定了19种与已知或假设的表皮蛋白（CP）同源的蛋白质，包括一种新的富含带电残基的低复杂性蛋白质。微阵列分析确定了372个基因的10倍或更大的差异，在鞘翅和后翅之间的转录水平。在鞘翅中具有较高表达的CP基因属于Rebers和Riddiford家族（CPR）2型，或富含甘氨酸或脯氨酸的低复杂性表皮蛋白（CPL C）。相比之下，大多数的CP基因与高表达的后翅被归类为CPR 1型，表皮蛋白类似于peritrophins（CPAP），或Tweedle家族的成员。这项研究表明，鞘翅和后翅，代表刚性和软cudeles，有不同的蛋白质和基因表达谱的结构蛋白，可能会影响这些cudeles的机械性能。
The insect cuticle is a composite biomaterial made up primarily of chitin) and proteins. The physical properties of the cuticle can vary greatly from hard and rigid to soft and flexible. Understanding how different cuticle types are assembled can aid in the development of novel biomimetic materials for use in medicine and technology. Toward this goal, we have taken a combined proteomics and transcriptomics approach with the red flour beetle, Tribolium castaneum, to examine the protein and gene expression profiles of the elytra and hindwings, appendages that contain rigid and soft cuticles, respectively. Two-dimensional gel electrophoresis analysis revealed distinct differences in the protein profiles between elytra and hindwings, with four highly abundant proteins dominating the elytral cuticle extract. MALDI/TOF mass spectrometry identified 19 proteins homologous to known or hypothesized cuticular proteins (CPs), including a novel low complexity protein enriched in charged residues. Microarray analysis identified 372 genes with a 10-fold or greater difference in transcript levels between elytra and hindwings. CP genes with higher expression in the elytra belonged to the Rebers and Riddiford family (CPR) type 2, or cuticular proteins of low complexity (CPLC) enriched in glycine or proline. In contrast, a majority of the CP genes with higher expression in hindwings were classified as CPR type 1, cuticular proteins analogous to peritrophins (CPAP), or members of the Tweedle family. This research shows that the elyra and hindwings, representatives of rigid and soft cuticles, have different protein and gene expression profiles for structural proteins that may influence the mechanical properties of these cuticles.