Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp tularensis
Characterization of the O antigen gene cluster and structural analysis of the O antigen of Francisella tularensis subsp tularensis
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DOI：
10.1099/jmm.0.05184-0
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发表时间：
2003-10-01
影响因子：
3
通讯作者：
Titball, RW
中科院分区：
文献类型：
--
作者：
Prior, JL;Prior, RG;Titball, RW
从土拉弗朗西斯菌（Francisella tularensis subsp.）的部分基因组序列中鉴定出一个编码参与LPS O抗原生物合成的酶的基因簇。土拉菌S4.基于与来自其他细菌的O抗原生物合成簇的基因的序列相似性，簇内的所有基因被分配推定的功能。设计了10对重叠引物，对9株F.土拉热。虽然基因簇存在于所有菌株，有一个大小的差异，在一个PCR产物之间的亚种。土拉菌菌株和土拉菌亚种全北极星菌株从F. tularensis subsp、tularensis Schu S4和O抗原通过质谱分析显示具有与F.土拉热亚种全北极线虫15.当LPS从F.土拉热亚种tularensis Schu S4用于免疫小鼠，然后用F.土拉热亚种tularensis Schu S4，观察到死亡时间延长。
A gene cluster encoding enzymes involved in LPS O antigen biosynthesis was identified from the partial genome sequence of Francisella tularensis subsp. tularensis Schu S4. All of the genes within the cluster were assigned putative functions based on sequence similarity with genes from O antigen biosynthetic clusters from other bacteria. Ten pairs of overlapping primers were designed to amplify the O antigen biosynthetic cluster by PCR from nine strains of F. tularensis. Although the gene cluster was present in all strains, there was a size difference in one of the PCR products between subsp. tularensis strains and subsp. holarctica strains. LPS was purified from F. tularensis subsp, tularensis Schu S4 and the O antigen was shown by mass spectrometry to have a structure similar to that of F. tularensis subsp. holarctica strain 15. When LPS from F. tularensis subsp. tularensis Schu S4 was used to immunize mice that were then challenged with F. tularensis subsp. tularensis Schu S4, an extended time to death was observed.