LYMPHOID DENDRITIC CELLS ARE POTENT STIMULATORS OF PRIMARY MIXED LEUKOCYTE REACTION IN MICE
LYMPHOID DENDRITIC CELLS ARE POTENT STIMULATORS OF PRIMARY MIXED LEUKOCYTE REACTION IN MICE
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DOI：
10.1073/pnas.75.10.5132
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
WITMER, MD
中科院分区：
文献类型：
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作者：
STEINMAN, RM;WITMER, MD
树突状细胞（DC）是一种新的细胞类型，最初在小鼠淋巴器官中发现。近年来，从小鼠脾脏中分离纯化了DC。本文证明了DC的功能作用：它们是原发性混合白细胞反应（MLR）的有效刺激物。少至300 - 1000个DC使5 × 10 - 6的增殖活性加倍。106个同种异体应答者脾细胞，而0.3 - 1.0 ×105 DC诱导的最大刺激为30 - 80倍。在这些极端之间，MLR响应的对数随DC数的对数线性增加。然后使用该剂量-反应测定来比较纯化的DC与其他异质淋巴样群体的效力，其中许多给出具有相似斜率的剂量-反应曲线。纯化的DC作为MLR刺激剂的效力比未分级的脾细胞的效力高100 - 200倍。当通过简单的物理技术分离脾细胞时，亚群中的MLR刺激能力与DC数量密切相关。通过抗免疫球蛋白或抗脑血清加补体去除脾B [骨髓来源]或T [胸腺来源]淋巴细胞不会降低MLR刺激能力。几个群体富含单核吞噬细胞，但缺乏DC刺激弱，如果在所有。DC是一种有效的刺激细胞，并且比其他主要细胞亚类，即，B和T淋巴细胞和巨噬细胞。
Dendritic cells (DC) are a new cell type initially identified in mouse lymphoid organs. Recently, DC were purified from mouse spleen. This paper demonstrates a functional role of DC: they are potent stimulators of the primary mixed leukocyte reaction (MLR). As few as 300-1000 DC doubled the proliferative activity of 5 .times. 106 allogeneic responder spleen cells, while 0.3-1.0 .times. 105 DC induced a maximal stimulation of 30- to 80-fold. Between these extremes, the log of the MLR response increased linearly with the log of DC numbers. This dose-response assay was then used to compare the potency of purified DCs with that of other heterogeneous lymphoid populations, many of which gave dose-response curves with similar slopes. The potency of purified DC as MLR stimulators was 100-200 times greater than that of unfractionated spleen cells. When spleen cells were fractionated by simple physical techniques, MLR-stimulating capacity in the subpopulations correlated closely with DC numbers. Removal of splenic B [bone marrow derived] or T [thymus derived] lymphocytes by anti-immunoglobulin or anti-brain serum plus complement did not reduce MLR-stimulating capacity. Several populations enriched in mononuclear phagocytes but lacking in DC stimulated weakly if at all. DC are a potent stimulating cell and are at least 100 times more effective than other major cell subclasses, i.e., B and T lymphocytes and macrophages.