ELECTRONIC SEPARATION OF BIOLOGICAL CELLS BY VOLUME
ELECTRONIC SEPARATION OF BIOLOGICAL CELLS BY VOLUME
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DOI：
10.1126/science.150.3698.910
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发表时间：
1965-01-01
期刊：
影响因子：
56.9
通讯作者：
FULWYLER, MJ
中科院分区：
文献类型：
--
作者：
FULWYLER, MJ
已经开发了一种能够根据体积分离生物细胞（悬浮在导电介质中）的装置。细胞体积在胶囊中测量，然后将细胞分离在介质的液滴中，该介质根据感应体积充电。然后，带电的液滴进入静电场，并将其偏转到收集容器中。小鼠和人红细胞的混合物以及小鼠淋巴瘤细胞的大量成分被成功分离。在与中国仓鼠卵巢细胞的测试中，所有细胞基本上都在分离并以正常速度生长。
A device capable of separating biological cells (suspended in a conducting medium) according to volume has been developed. Cell volume is measured in a Coulter aperture, and the cells are subsequently isolated in droplets of the medium which are charged according to the sensed volume. The charged droplets then enter an electrostatic field and are deflected into a collection vessel. Mixtures of mouse and human erythrocytes and a large volume component of mouse lymphoma cells were separated successfully. In tests with Chinese hamster ovary cells essentially all cells survived separation and grew at their normal rate.