Comparison of Cas9 activators in multiple species.
Comparison of Cas9 activators in multiple species.
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DOI：
10.1038/nmeth.3871
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发表时间：
2016-07
期刊：
影响因子：
48
通讯作者：
Church G
中科院分区：
文献类型：
--
作者：
Chavez A;Tuttle M;Pruitt BW;Ewen-Campen B;Chari R;Ter-Ovanesyan D;Haque SJ;Cecchi RJ;Kowal EJK;Buchthal J;Housden BE;Perrimon N;Collins JJ;Church G
有几个小组已经产生了基于多功能Cas9蛋白的可编程转录因子，但它们在不同细胞类型和物种中的相对效力和有效性仍未被探索。在这里，我们比较了Cas9激活系统，并检测了它们在几个人、小鼠和苍蝇细胞系中诱导强劲基因表达的能力。我们还探索了通过组合最有效的激活系统来改善激活的可能性，并评估了协作性在最大化基因表达方面的作用。
Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems and assess the role of cooperativity in maximizing gene expression.