Non-homologous end joining: advances and frontiers.
Non-homologous end joining: advances and frontiers.
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DOI：
10.1093/abbs/gmw046
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发表时间：
2016-07
影响因子：
3.7
通讯作者：
Kai Yang;R. Guo;Dongyi Xu
中科院分区：
文献类型：
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作者：
Kai Yang;R. Guo;Dongyi Xu
DNA双链断裂(DSB)是DNA损伤最严重的形式。在人类细胞中，非同源末端连接(NHEJ)是双链断裂修复的主要途径。不同类型的DSB导致NHEJ修复策略的不同子集。NHEJ修复策略的这些变化取决于许多因素，例如NHEJ相关蛋白的灵活招募、DSB末端的复杂性以及多蛋白复合体的空间和时间有序形成。一方面，目前对DNA双链断裂修复的研究主要集中在同源重组和经典或替代NHEJ修复途径的选择上。另一方面，越来越多的研究也加深了NHEJ途径的意义，并深入挖掘了NHEJ途径与基因组组织和衰老领域之间的交叉联系。虽然在过去的几十年里，在阐明基本原理方面已经取得了显著的进展，但对不同类型DSB的具体作用机制仍然很大程度上是未知的，需要在未来的研究中进一步评估。
DNA double-strand breaks (DSBs) are the most serious form of DNA damage. In human cells, non-homologous end joining (NHEJ) is the major pathway for the repair of DSBs. Different types of DSBs result in different subsets of NHEJ repair strategies. These variations in NHEJ repair strategies depend on numerous elements, such as the flexible recruitment of NHEJ-related proteins, the complexity of the DSB ends, and the spatial- and temporal-ordered formation of the multi-protein complex. On the one hand, current studies of DNA DSBs repair focus on the repair pathway choices between homologous recombination and classic or alternative NHEJ. On the other hand, increasing researches have also deepened the significance and dug into the cross-links between the NHEJ pathway and the area of genome organization and aging. Although remarkable progress has been made in elucidating the underlying principles during the past decades, the detailed mechanism of action in response to different types of DSBs remains largely unknown and needs further evaluation in the future study.