Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway.
Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPARγ pathway.
复制标题
DOI：
10.1080/21623945.2021.1906509
复制
发表时间：
2021-12
期刊：
影响因子：
3.3
通讯作者：
Lin M
中科院分区：
文献类型：
--
作者：
Qian Y;Chen H;Pan T;Li T;Zhang Z;Lv X;Wang J;Ji Z;He Y;Li L;Lin M
脂肪源性干细胞（ADSCs）的高活力和进一步的成脂分化是移植脂肪组织移植和生长的基础。研究表明，细胞外基质（extracellular matrix， ECM）通过与ERK1/2信号通路相互作用调控细胞增殖和分化。本研究制备了自体脱细胞细胞外基质（d-ECM），并探讨其对低血清培养ADSCs增殖和成脂能力的影响。我们发现，与10%胎牛血清相比，2%胎牛血清在生长培养基中抑制了细胞活力和DNA复制，降低了PPARγ和C/EPBα mRNA和蛋白水平。相应的，在2%胎牛血清中培养的细胞在诱导成脂14天后，其成脂效率较低，脂肪细胞分化标志物ADIPOQ和aP2表达较少。相反，d- ecm包被的底物在分化过程中不断促进PPARγ的表达，并以不同方式调控ERK1/2的磷酸化。用ERK1/2抑制剂PD98059预处理可中和d-ECM的作用，提示d-ECM可能通过ERK1/2- ppar γ途径调节ADSCs的脂肪形成。此外，d-ECM在未分化的ADSCs中调控OCT4、NANOG和SOX2等干细胞相关基因的转录和表达，这可能与分化的开始有关。
High viability and further adipogenic differentiation of adipose-derived stem cells (ADSCs) are fundamental for engraftment and growth of the transplanted adipose tissue. It has been demonstrated that extracellular matrix (ECM) regulates cell proliferation and differentiation by interacting with ERK1/2 signalling pathway. In this study, we prepared autologous decellularized extracellular matrix (d-ECM) and explored its effect on the proliferation and adipogenic ability of ADSCs in low serum culture. We found that 2% foetal bovine serum (FBS) in growth medium inhibited cell viability and DNA replication, and decreased mRNA and protein levels of PPARγ and C/EPBα compared with 10% FBS. Correspondingly, after 14-days adipogenic induction, cells cultured in 2% FBS possessed lower efficiency of adipogenesis and expressed less adipocyte differentiation markers ADIPOQ and aP2. On the contrary, the d-ECM-coated substrate continuously promoted the expression of PPARγ, and regulated the phosphorylation of ERK1/2 in different manners during differentiation. Pretreatment with ERK1/2 inhibitor PD98059 neutralized the effects of d-ECM, which suggested d-ECM might regulate the adipogenesis of ADSCs through ERK1/2-PPARγ pathway. In addition, d-ECM was revealed to regulate the transcription and expression of stemness-associated genes, such as OCT4, NANOG and SOX2, in the undifferentiated ADSCs, which might be related to the initiation of differentiation.
登录
查看更多内容
影响因子：
4.1
作者：
Bost, F;Caron, L;Binétruy, B
通讯作者：
Binétruy, B
影响因子：
3.3
作者：
Kim, Jae Hyung;Shin, Sang-Hyun;Suh, Hwal
通讯作者：
Suh, Hwal
影响因子：
--
作者：
MELOCHE, S;SEUWEN, K;POUYSSEGUR, J
通讯作者：
POUYSSEGUR, J
影响因子：
8.8
作者：
Pogrmic-Majkic, Kristina;Nenadov, Dragana Samardzija;Andric, Nebojsa
通讯作者：
Andric, Nebojsa
影响因子：
5.3
作者：
deMora, JF;Porras, A;Santos, E
通讯作者：
Santos, E