Guanine Can Direct Binding Specificity of Ru-dipyridophenazine (dppz) Complexes to DNA through Steric Effects.
Guanine Can Direct Binding Specificity of Ru-dipyridophenazine (dppz) Complexes to DNA through Steric Effects.
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DOI：
10.1002/chem.201605508
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发表时间：
2017-04-11
期刊：
影响因子：
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通讯作者：
Cardin CJ
中科院分区：
文献类型：
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作者：
Hall JP;Gurung SP;Henle J;Poidl P;Andersson J;Lincoln P;Winter G;Sorensen T;Cardin DJ;Brazier JA;Cardin CJ
通过X-射线衍射分析，确定了3个配合物（dppz= dipridophenazine; L= 1，10-phen，2，2 ′-bpy）与d（（5 BrC）GGC/GCCG）的插层方式为5′-CG-3′阶。化合物通过倾斜嵌入结合，结合角由鸟嘌呤NH 2基团决定，与之前在5′-TA-3′位点观察到的对称嵌入相反。这一结果表明，即使5′-CG-3′位置本身是对称的，倾斜嵌入也是优选的，并且我们假设在5′-CG-3′步骤中的对称嵌入可以产生比倾斜嵌入更长的发光寿命。
X‐ray crystal structures of three Λ‐[Ru(L)2dppz]2+ complexes (dppz=dipyridophenazine; L=1,10‐phenanthroline (phen), 2,2′‐bipyridine (bpy)) bound to d((5BrC)GGC/GCCG) showed the compounds intercalated at a 5′‐CG‐3′ step. The compounds bind through canted intercalation, with the binding angle determined by the guanine NH2 group, in contrast to symmetrical intercalation previously observed at 5′‐TA‐3′ sites. This result suggests that canted intercalation is preferred at 5′‐CG‐3′ sites even though the site itself is symmetrical, and we hypothesise that symmetrical intercalation in a 5′‐CG‐3′ step could give rise to a longer luminescence lifetime than canted intercalation.