Chemical and structural effects of base modifications in messenger RNA.
Chemical and structural effects of base modifications in messenger RNA.
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DOI：
10.1038/nature21351
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发表时间：
2017-01-18
期刊：
影响因子：
64.8
通讯作者：
Kool ET
中科院分区：
文献类型：
--
作者：
Harcourt EM;Kietrys AM;Kool ET
通过检测和RNA测序的进展，已经揭示了信使RNA中越来越多的核碱基修饰。虽然已经确定了一些涉及修饰碱基的生化途径，但对RNA修饰世界的研究-表转录组-仍处于早期阶段。各种化学工具被用来表征碱基修饰，已知的碱基修饰对RNA配对，热力学和折叠的结构影响正在确定与其假定的生物学作用。
A growing number of nucleobase modifications in messenger RNA have been revealed through advances in detection and RNA sequencing. Although some of the biochemical pathways that involve modified bases have been identified, research into the world of RNA modification — the epitranscriptome — is still in an early phase. A variety of chemical tools are being used to characterize base modifications, and the structural effects of known base modifications on RNA pairing, thermodynamics and folding are being determined in relation to their putative biological roles.