Shroom family proteins regulate γ-tubulin distribution and microtubule architecture during epithelial cell shape change
Shroom family proteins regulate γ-tubulin distribution and microtubule architecture during epithelial cell shape change
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DOI：
10.1242/dev.02828
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发表时间：
2007-04-01
期刊：
影响因子：
4.6
通讯作者：
Wallingford, John B.
中科院分区：
文献类型：
--
作者：
Lee, Chanjae;Scherr, Heather M.;Wallingford, John B.
细胞形状的改变需要肌动蛋白和微管细胞骨架的协调。实现这种协调的分子机制仍然不清楚，特别是在脊椎动物胚胎发育中的上皮细胞的背景下。我们已经确定了肌动蛋白结合蛋白 Shroom3 在上皮形态发生过程中作为微管细胞骨架调节剂的新作用。我们证明 Shroom3 对于诱导微管调节剂 γ-微管蛋白的重新分布是充分且必要的。此外，γ-微管蛋白分布的这种变化是驱动顶端基底细胞伸长的微管对齐阵列组装的基础。最后，相关蛋白 Shroom1 的实验表明，γ-微管蛋白调节是该蛋白家族的保守特征。总之，数据表明 Shroom 家族蛋白通过协调微管和肌动蛋白细胞骨架的组装来控制上皮细胞行为。
Cell shape changes require the coordination of actin and microtubule cytoskeletons. The molecular mechanisms by which such coordination is achieved remain obscure, particularly in the context of epithelial cells within developing vertebrate embryos. We have identified a novel role for the actin-binding protein Shroom3 as a regulator of the microtubule cytoskeleton during epithelial morphogenesis. We show that Shroom3 is sufficient and also necessary to induce a redistribution of the microtubule regulator gamma-tubulin. Moreover, this change in gamma-tubulin distribution underlies the assembly of aligned arrays of microtubules that drive apicobasal cell elongation. Finally, experiments with the related protein, Shroom1, demonstrate that gamma-tubulin regulation is a conserved feature of this protein family. Together, the data demonstrate that Shroom family proteins govern epithelial cell behaviors by coordinating the assembly of both microtubule and actin cytoskeletons.